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. 2024 May 20;49(2):122-129.
doi: 10.1584/jpestics.D24-009.

Field survey of reproductive modes and sodium channel mutations associated with pyrethroid resistance in Thrips tabaci

Yui Tomizawa  1 , Misato Aizawa  2 , Akiya Jouraku  3 , Shoji Sonoda  1
Affiliations

Field survey of reproductive modes and sodium channel mutations associated with pyrethroid resistance in Thrips tabaci

Yui Tomizawa et al. J Pestic Sci. .

Abstract

Using PCR-Restriction Fragment Length Polymorphism (RFLP) with mitochondrial cytochrome c oxidase subunit I sequences, we examined the reproductive modes of female adults of Thrips tabaci collected at 54 sites across Japan. Results showed the presence of heteroplasmic insects harboring mitochondria associated with arrhenotoky and thelytoky. Using the insects, we also applied PCR-RFLP to examine the genotypes for the amino acid mutation (T929I) site involved in pyrethroid resistance. Findings showed the presence of thelytokous heterozygotes under the circumstance that most arrhenotokous insects are resistant homozygotes, and many thelytokous insects are susceptible homozygotes. These results suggest that, in the field, genetic exchange occurs between insects through of both reproductive modes. A survey of the genotypes for the other amino acid mutations using nucleotide sequencing showed a decline of insects with an M918T and L1014F pair and an increase of insects with M918L. These results suggest the evolutional progression of amino acid mutations associated with pyrethroid resistance in T. tabaci.

Keywords: arrhenotoky; insecticide resistance; onion thrips; target insensitivity; thelytoky.

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Figures

None
Fig. 1. Restriction patterns of the COI gene fragments amplified from Thrips tabaci. After the COI gene fragments amplified by PCR were digested with EcoO109I, they were size fractionated on 2% agarose gel. Restriction patterns for arrhenotoky and thelytoky were observed, respectively, in lanes 3, 4, and 5 and in lanes 6, 8, and 9. In lanes 1, 2, and 7, restriction patterns for both reproductive modes were observed.
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