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. 1985 May 10;79(1):99-108.
doi: 10.1016/0022-1759(85)90396-5.

A monoclonal antibody based solid-phase enzyme-binding assay to measure levels of placental alkaline phosphatase in serum of women during pregnancy

S F Contractor, E Holmes-Ievers, A Morgan, M Oakey, N A Staines

A monoclonal antibody based solid-phase enzyme-binding assay to measure levels of placental alkaline phosphatase in serum of women during pregnancy

S F Contractor et al. J Immunol Methods. .

Abstract

Monoclonal antibodies raised against highly purified placental alkaline phosphatase (PALP) reacted specifically with PALP and were found not to cross-react with the liver, kidney and intestinal enzymes. One of these antibodies (AP-3) was selected to develop an enzyme-binding assay to measure levels of PALP in the sera of 11 women throughout pregnancy. PALP activity rose sharply after 28 weeks of gestation reaching a peak at delivery followed by an immediate drop within 4 days to values approaching non-pregnant levels. The potential of using this assay as an index of placental function to monitor progress of a pregnancy is discussed.

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