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. 2024 May 24;13(11):3080.
doi: 10.3390/jcm13113080.

Evaluation of Regulatory B Cell Subpopulations CD24++CD38++, CD24++CD27+, Plasmablasts and Their Correlation with T Regs CD3+CD4+CD25+FOXP3+ in Dialysis Patients and Early Post-Transplant Rejection-Free Kidney Recipients

Ariadni Fouza  1 , Asimina Fylaktou  2 , Anneta Tagkouta  3   4 , Maria Daoudaki  3 , Lampros Vagiotas  1 , Efstratios Kasimatis  5 , Maria Stangou  5 , Aliki Xochelli  2 , Vasiliki Nikolaidou  2 , Georgios Katsanos  1 , Georgios Tsoulfas  1 , Lemonia Skoura  6 , Aikaterini Papagianni  5 , Nikolaos Antoniadis  1
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Evaluation of Regulatory B Cell Subpopulations CD24++CD38++, CD24++CD27+, Plasmablasts and Their Correlation with T Regs CD3+CD4+CD25+FOXP3+ in Dialysis Patients and Early Post-Transplant Rejection-Free Kidney Recipients

Ariadni Fouza et al. J Clin Med. .

Abstract

Background: B and T regulatory cells, also known as Bregs and Tregs, are involved in kidney transplantation. The purpose of this study is to monitor changes in the frequency and absolute numbers of Tregs (CD3+CD4+CD25+FoxP3+), transitional Bregs (tBregs) (CD24++CD38++), memory Bregs (mBregs) (CD24++CD27+), and plasmablasts before (T0) and six months (T6) after transplantation. Additionally, we aim to investigate any correlation between Tregs and tBregs, mBregs, or plasmablasts and their relationship with graft function. Methods: Flow cytometry was used to immunophenotype cells from 50 kidney recipients who did not experience rejection. Renal function was assessed using the estimated glomerular filtration rate (eGFR). Results: At T6, there was a significant decrease in the frequency of Tregs, plasmablasts, and tBregs, as well as in the absolute number of tBregs. The frequency of mBregs, however, remained unchanged. Graft function was found to have a positive correlation with the frequency of tBregs and plasmablasts. A significant correlation was observed between the frequency and absolute number of tBregs only when the eGFR was greater than 60 but not at lower values. At an eGFR greater than 60, there was a positive correlation between the absolute numbers of Tregs and mBregs but not between Tregs and tBregs. No correlation was observed for any cell population in dialysis patients. Conclusions: The data show a correlation between the frequency and absolute number of tBregs and the absolute number of Tregs and mBregs with good renal function in the early post-transplant period.

Keywords: CD24++CD27+ (mBregs); CD24++CD38++ (tBregs); CD3+CD4+CD25+FoxP3+ (Tregs); kidney transplantation; plasmablasts.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Gating strategy for B cell subpopulations. Representative flow cytometric data. CD45 and side scatter gating for lymphocytes. Total B cells are CD19+ cells, which are then analyzed for tBregs and mBregs cells.
Figure 2
Figure 2
Correlation matrix between the absolute numbers (no) and frequency of mBregs and Tregs of transplanted patients at T6 with (a) eGFR ≤ 40 and (b) eGFR ≥ 60. A significant correlation is shown with an asterisk.
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