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. 1985 Apr 25;13(8):2789-802.
doi: 10.1093/nar/13.8.2789.

A complex of single-strand binding protein and M13 DNA as hybridization probe

A C Syvänen, M Alanen, H Söderlund
Free PMC article

A complex of single-strand binding protein and M13 DNA as hybridization probe

A C Syvänen et al. Nucleic Acids Res. .
Free PMC article

Abstract

Single-stranded DNA was complexed to the single-strand binding protein (SSB) of Escherichia coli in a mass ratio of 30:1. The protein moiety of this complex can be labelled by a number of methods of which we have chosen radio-iodination and biotinylation as examples. The SSB-M13 DNA complexes, labelled to high specific activities, were used as probes in hybridization experiments in which 1.6 X 10(-18) moles of immobilized target DNA were detected. The stability of the hybrids was not severely decreased by the binding of SSB. Analysis of hybrids by electron microscopy showed that complexing of DNA with SSB could be used to allow its subsequent identification in the hybrids.

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References

    1. Annu Rev Biophys Bioeng. 1978;7:19-35 - PubMed
    1. Cell. 1975 May;5(1):45-50 - PubMed
    1. Nucleic Acids Res. 1979 Nov 24;7(6):1541-52 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4274-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6633-7 - PubMed