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. 2025 Aug;63(4):3187-3205.
doi: 10.1007/s10528-024-10849-8. Epub 2024 Jun 24.

Exploring of miR-155-5p, miR-181b-5p, and miR-454-3p Expressions in Circulating Cell-Free RNA: Insights from Peripheral Blood of Uveal Malignant Melanoma Patients

Hassani Masoumeh  1 , Doğan Tunay  1   2 , Ödemiş Akdeniz Demet  1   3 , Tuncer Samuray  4 , Yazıcı Hülya  5   6
Affiliations

Exploring of miR-155-5p, miR-181b-5p, and miR-454-3p Expressions in Circulating Cell-Free RNA: Insights from Peripheral Blood of Uveal Malignant Melanoma Patients

Hassani Masoumeh et al. Biochem Genet. 2025 Aug.

Abstract

The identification of novel non-invasive biomarkers is imperative for the early diagnosis and monitoring of malignant melanoma. The objective of this study is to examine the expression levels of miR-155-5p, miR-181b-5p, and miR-454-3p in circulating cell-free RNA obtained from plasma samples of the 72 uveal malignant melanoma patients and to compare these levels with those of 72 healthy controls. The analysis showed that the expression level of the miR-181b-5p has increased 9.25 fold, and expression level of miR-155-5p has increased 6.67 fold, and miR-454-3p expression level has increased 4.14 fold in the patient group compared with the levels in the healthy control group (p = 0.005). It was found that the high expression levels of the three miRNAs were statistically significant in patients compared with in the healthy control group. The statistical evaluations between miRNA expression levels and clinical data showed that miR-155-5p had significant association with radiation therapy (p = 0.040), and miR-454-3p showed a significant association with smoking and alcohol use respectively (p = 0.009, and p = 0.026). The significantly elevated expression levels of miR-181b-5p, miR-155-5p, and miR-454-3p in the circulating cell-free RNA of plasma from uveal melanoma patients, in comparison to those in the healthy control group, suggest the potential usefulness of these biomarkers for both early diagnosis and disease monitoring. However, more extensive and future studies are needed to use these molecules in early diagnosis and disease monitoring.

Keywords: Circulating cell free miRNA; Plasma; Uveal Melanoma; miR-155-5p; miR-181b-5p; miR-454-3p.

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Conflict of interest statement

Declarations. Competing interests: The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
The comparison of the expression levels of miR-181b-5p, miR-155-5p and miR-454-3p in uveal melanoma patients with the healthy control group
Fig. 2
Fig. 2
STRING analysis for target genes of miR-181b-5p (p value: 0.000118)
Fig. 3
Fig. 3
STRING analysis for target genes of miR-155-5p (p value: 0.000399)
Fig. 4
Fig. 4
STRING analysis for target genes of miR-454-3p (p value: 0.000129)
Fig. 5
Fig. 5
ROC analysis curve of miR-181b-5p, miR-155-5p and miR-454-3p
See this image and copyright information in PMC

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