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. 1985 Jul;163(1):349-54.
doi: 10.1128/jb.163.1.349-354.1985.

Mini-F protein that binds to a unique region for partition of mini-F plasmid DNA

Y Hayakawa, T Murotsu, K Matsubara

Mini-F protein that binds to a unique region for partition of mini-F plasmid DNA

Y Hayakawa et al. J Bacteriol. 1985 Jul.

Abstract

A mini-F-coded protein, named F2 protein, binds specifically to mini-F DNA. This protein has a molecular weight of 37,000 and is coded by the A2 segment of the mini-F genome (47.3 to 49.4 kilobases on the F coordinate map). The binding site is located also in the A2 segment of mini-F. This binding site is lost by spontaneous deletion when the A2 segment alone, but not A2 together with its neighboring segment, is cloned in a multicopy plasmid pBR322. These data are discussed in connection with incompatibility and plasmid stability.

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