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Comparative Study
. 1985 Jun 12;80(1):45-53.
doi: 10.1016/0022-1759(85)90163-2.

MicroELISA method for measurement of human serum thymosin alpha 1

F E Weller, M G Mutchnick, D F Keren, A L Goldstein, P H Naylor
Comparative Study

MicroELISA method for measurement of human serum thymosin alpha 1

F E Weller et al. J Immunol Methods. .

Abstract

A microELISA for the estimation of human serum thymosin alpha 1 is described. In this assay, antibody to thymosin alpha 1 is pre-incubated with the standard or serum at 4 degrees C. Unbound antibody in the liquid-phase then binds with solid-phase thymosin alpha 1. The method is sufficiently sensitive for measuring serum levels of thymosin alpha 1 and highly reproducible. The serum levels measured with the microELISA are comparable to serum levels of thymosin alpha 1 determined by the previously described radioimmunoassay for thymosin alpha 1.

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