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. 2024 Jun 12;10(3):35.
doi: 10.3390/ncrna10030035.

The miRNA Contribution in Adipocyte Maturation

Alessandro Giammona  1   2   3 , Simone Di Franco  3 , Alessia Lo Dico  1   2 , Giorgio Stassi  3
Affiliations

The miRNA Contribution in Adipocyte Maturation

Alessandro Giammona et al. Noncoding RNA. .

Abstract

Mesenchymal stem cells, due to their multipotent ability, are considered one of the best candidates to be used in regenerative medicine. To date, the most used source is represented by the bone marrow, despite the limited number of cells and the painful/invasive procedure for collection. Therefore, the scientific community has investigated many alternative sources for the collection of mesenchymal stem cells, with the adipose tissue representing the best option, given the abundance of mesenchymal stem cells and the easy access. Although adipose mesenchymal stem cells have recently been investigated for their multipotency, the molecular mechanisms underlying their adipogenic potential are still unclear. In this scenario, this communication is aimed at defining the role of miRNAs in adipogenic potential of adipose-derived mesenchymal stem cells via real-time PCR. Even if preliminary, our data show that cell culture conditions affect the expression of specific miRNA involved in the adipogenic potential of mesenchymal stem cells. The in vitro/in vivo validation of these results could pave the way for novel therapeutic strategies in the field of regenerative medicine. In conclusion, our research highlights how specific cell culture conditions can modulate the adipogenic potential of adipose mesenchymal stem cells through the regulation of specific miRNAs.

Keywords: adipose stem cells; adipose tissue; mesenchymal stem cells (MSC); miRNA; regenerative medicine.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest.

Figures

Figure 1
Figure 1
Mesenchymal stem cell commitment. The differentiation of mesenchymal stem cells into mature adipocytes involves both a preliminary lineage commitment and subsequent terminal differentiation.
Figure 2
Figure 2
miRNA families that regulate specific target genes involved in adipogenesis.
Figure 3
Figure 3
The differences in terms of multi-difference efficiency could be due to miRNA expression. (A) Clustergrams of mesenchymal stem cell and their derived mature adipocytes, ADA (ADSC-derived adipocytes) and SDA (ASphC-derived adipocytes). The score index is expressed using equivalent Cт values, where the original Cт values are projected to 100% target efficiency. (B) Scatter plot of human miRNA expression, and miRNA expression reported as fold change ADSCs compared with ASphCs.
Figure 4
Figure 4
The miRNAs acting on different pathways. (A) Scatter plot of human microRNA expressed by mesenchymal stem cell ASphCs and their derived mature adipocytes, SDA (ASphC-derived adipocytes). (B) Scatter plots of human microRNA expressed by mesenchymal stem cell ADSC and their derived mature adipocytes, ADA (ASphC-derived adipocytes).
Figure 5
Figure 5
Graphic synthesis of comparative analysis between the miRNAs described in the literature and the miRNAs observed from experimental data.
See this image and copyright information in PMC

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