Gene assignments of a cytoplasmic polyhedrosis virus (type 2) from Nymphalis io

Abstract

The 10 double stranded (ds) RNAs from cytoplasmic polyhedrosis virus (type 2) from Nymphalis io have been separated by polyacrylamide gel electrophoresis and fractionated into separate genes. Separate and total dsRNA were denatured using methyl mercury, and translated in a rabbit reticulocyte lysate. Translation of unfractionated RNA generated proteins that comigrated with virion and polyhedral structural proteins and six major non-structural proteins. Translation of individual RNAs, demonstrated that the virion structural proteins were coded for by the largest RNAs and the polyhedral protein by the smallest genome segment. RNAs 7 and 8 each produced two major non-structural proteins and no primary product was found for RNA 3. Reaction of the translation products with antiserum raised against purified virions immunoprecipitated putative structural proteins (and some "non-structural"). Antiserum against the polyhedral protein immunoprecipitated "non-structural" proteins and the polyhedral protein, but not virus particle structural proteins.