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. 2024 Sep;47(9):e13985.
doi: 10.1111/jfd.13985. Epub 2024 Jun 24.

Replacement of fishmeal with a microbial single-cell protein induced enteropathy and poor growth outcomes in barramundi (Lates calcarifer) fry

Francisca Samsing  1 , Roisin Sullivan  1   2 , Ha Truong  3 , Artur Rombenso  3 , Cheryl R Sangster  4 , Jo Bannister  5 , Matt Longshaw  6 , Joy A Becker  2
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Replacement of fishmeal with a microbial single-cell protein induced enteropathy and poor growth outcomes in barramundi (Lates calcarifer) fry

Francisca Samsing et al. J Fish Dis. 2024 Sep.

Abstract

Fish meal (FM) replacement is essential for the sustainable expansion of aquaculture. This study focussed on the feasibility of replacing FM with a single-cell protein (SCP) derived from methanotrophic bacteria (Methylococcus capsulatus, Bath) in barramundi fry (Lates calcarifer). Three isonitrogenous and isoenergetic diets were formulated with 0%, 6.4% and 12.9% inclusion of the SCP, replacing FM by 0%, 25% and 50%. Barramundi fry (initial body weight 2.5 ± 0.1 g) were fed experimental diets for 21 days to assess growth performance, gut microbiome composition and gut histopathology. Our findings revealed that both levels of SCP inclusion induced detrimental effects in barramundi fry, including impaired growth and reduced survival compared with the control group (66.7% and 71.7% survival in diets replacing FM with SCP by 25% and 50%, respectively; p < .05). Both dietary treatments presented mild necrotizing enteritis with subepithelial oedema and accumulation of PAS positive, diastase resistant droplets within hepatocytes (ceroid hepatopathy) and pancreatic atrophy. Microbiome analysis revealed a marked shift in the gut microbial community with the expansion of potential opportunistic bacteria in the genus Aeromonas. Reduced overall performance in the highest inclusion level (50% SCP) was primarily associated with reduced feed intake, likely related to palatability issues, albeit pathological changes observed in gut and liver may also play a role. Our study highlights the importance of meticulous optimization of SCP inclusion levels in aquafeed formulations, and the need for species and life-stage specific assessments to ensure the health and welfare of fish in sustainable aquaculture practices.

Keywords: alternative protein ingredients; aquaculture sustainability; aquafeeds; fish health and welfare; fish nutrition; nutritional disease.

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References

REFERENCES

    1. Øverland, M., Tauson, A. H., Shearer, K., & Skrede, A. (2010). Evaluation of methane‐utilising bacteria products as feed ingredients for monogastric animals. Archives of Animal Nutrition, 64(3), 171–189. https://doi.org/10.1080/17450391003691534
    1. Aas, T. S., Grisdale‐Helland, B., Terjesen, B. F., & Helland, S. J. (2006). Improved growth and nutrient utilisation in Atlantic salmon (Salmo salar) fed diets containing a bacterial protein meal. Aquaculture, 259(1), 365–376. https://doi.org/10.1016/j.aquaculture.2006.05.032
    1. Aas, T. S., Hatlen, B., Grisdale‐Helland, B., Terjesen, B. F., Penn, M., Bakke‐McKellep, A. M., & Helland, S. J. (2007). Feed intake, growth and nutrient utilization in Atlantic halibut (Hippoglossus hippoglossus) fed diets containing a bacterial protein meal. Aquaculture Research, 38(4), 351–360. https://doi.org/10.1111/j.1365‐2109.2007.01672.x
    1. Agboola, J. O., Chikwati, E. M., Hansen, J. Ø., Kortner, T. M., Mydland, L. T., Krogdahl, Å., Djordjevic, B., Schrama, J. W., & Øverland, M. (2022). A meta‐analysis to determine factors associated with the severity of enteritis in Atlantic salmon (Salmo salar) fed soybean meal‐based diets. Aquaculture, 555, 738214. https://doi.org/10.1016/j.aquaculture.2022.738214
    1. Biswas, A., Takakuwa, F., Yamada, S., Furukawa, A., Shiratori, M., Koshiishi, T., Tomokane, H., & Tanaka, H. (2021). Partial replacement of fishmeal by a protein‐rich product from methanotrophic bacteria in juvenile red sea bream (Pagrus major). Aquaculture Nutrition, 27(6), 2726–2738. https://doi.org/10.1111/anu.13398

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