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. 2024:2810:161-180.
doi: 10.1007/978-1-0716-3878-1_11.

Expression of Multispecific Antibodies

Megane Peltret  1 , Angelique Schmid  1 , Lionel Duarte  1 , Romain Mette  1 , Roberto Giovannini  1 , Martin Bertschinger  2
Affiliations

Expression of Multispecific Antibodies

Megane Peltret et al. Methods Mol Biol. 2024.

Abstract

Bi- and multispecific antibody formats allow the development of new therapeutic strategies to address previously unmet medical needs. However, due to the increased complexity (e.g., the interface design and the presence of multiple binders), such molecules are generally more challenging to express and purify compared to standard monoclonal antibodies (mAbs). We describe here an optimized methodology to express and purify basic bispecific antibodies using the BEAT® interface. This interface allows to generate antibodies with very high levels of heterodimer product (reported titers exceed 10 g/L) and comes with a built-in purification strategy allowing removal of residual levels of undesired product-related impurities (e.g., homodimers and half molecules).

Keywords: BEAT; Bioreactor process; Bispecific antibody; Downstream processing; Expression vector; Multispecific antibody; Stable expression.

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