Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2024 Jun 1;15(6):726.
doi: 10.3390/genes15060726.

In Search of a Target Gene for a Desirable Phenotype in Aquaculture: Genome Editing of Cyprinidae and Salmonidae Species

Svetlana Yu Orlova  1 , Maria N Ruzina  1 , Olga R Emelianova  1   2 , Alexey A Sergeev  1 , Evgeniya A Chikurova  1 , Alexei M Orlov  3   4   5 , Nikolai S Mugue  1   6
Affiliations
Review

In Search of a Target Gene for a Desirable Phenotype in Aquaculture: Genome Editing of Cyprinidae and Salmonidae Species

Svetlana Yu Orlova et al. Genes (Basel). .

Abstract

Aquaculture supplies the world food market with a significant amount of valuable protein. Highly productive aquaculture fishes can be derived by utilizing genome-editing methods, and the main problem is to choose a target gene to obtain the desirable phenotype. This paper presents a review of the studies of genome editing for genes controlling body development, growth, pigmentation and sex determination in five key aquaculture Salmonidae and Cyprinidae species, such as rainbow trout (Onchorhynchus mykiss), Atlantic salmon (Salmo salar), common carp (Cyprinus carpio), goldfish (Carassius auratus), Gibel carp (Carassius gibelio) and the model fish zebrafish (Danio rerio). Among the genes studied, the most applicable for aquaculture are mstnba, pomc, and acvr2, the knockout of which leads to enhanced muscle growth; runx2b, mutants of which do not form bones in myoseptae; lepr, whose lack of function makes fish fast-growing; fads2, Δ6abc/5Mt, and Δ6bcMt, affecting the composition of fatty acids in fish meat; dnd mettl3, and wnt4a, mutants of which are sterile; and disease-susceptibility genes prmt7, gab3, gcJAM-A, and cxcr3.2. Schemes for obtaining common carp populations consisting of only large females are promising for use in aquaculture. The immobilized and uncolored zebrafish line is of interest for laboratory use.

Keywords: CRISPR/Cas9; Carassius auratus cuvieri; Carassius gibelio; Cyprinus carpio; Danio rerio; Oncorhynchus mykiss; Salmo salar; TALEN; ZFN.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Number of articles on zebrafish genome editing in the PubMed article database from 2008 to 2023 using ZFN, TALEN, and CRISPR/Cas9 technologies.
See this image and copyright information in PMC

References

    1. The State of Food and Agriculture 2020. FAO; Rome, Italy: 2020.
    1. The State of World Fisheries and Aquaculture 2022. FAO; Rome, Italy: 2022.
    1. Mair G., Lucente D. What are “farmed types” in aquaculture and why do they matter? FAO Aquac. Newsl. 2020;61:40–42.
    1. Donaldson L.R., Olson P.R. Development of rainbow trout brood stock by selective breeding. Trans. Am. Fish. Soc. 1957;85:93–101. doi: 10.1577/1548-8659(1955)85[93:DORTBS]2.0.CO;2. - DOI
    1. Thodesen J., Gjedrem T. Breeding programs on Atlantic salmon in Norway: Lessons learned . [(accessed on 28 May 2024)];RePec. 2006 :22–26. Available online: https://digitalarchive.worldfishcenter.org/bitstream/handle/20.500.12348....