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Review
. 2021 Dec 24;2(1):56-58.
doi: 10.1016/j.fmre.2021.11.034. eCollection 2022 Jan.

Chemical labelling for m6A detection: opportunities and challenges

Affiliations
Review

Chemical labelling for m6A detection: opportunities and challenges

Yafen Wang et al. Fundam Res. .

Abstract

Image, graphical abstract.

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Conflict of interest statement

The authors declare that they have no conflicts of interest in this work.

Figures

Image, graphical abstract
Graphical abstract
Fig 1
Fig. 1
(a) Illustration of the formation of dm6A. (b) Schematic diagram of m6A-SEAL.
Fig 2
Fig. 2
(a) Illustration of the deamination of A. (b) Schematic diagram of NOseq,.
Fig 3
Fig. 3
(a) Illustration of the formation of cyc-A. (b) Schematic diagram of m6A-label-seq.

References

    1. Barbieri I., Tzelepis K., Pandolfini L., et al. Promoter-bound METTL3 maintains myeloid leukaemia by m6A-dependent translation control. Nature. 2017;552(7683):126–131. - PMC - PubMed
    1. Yankova E., Blackaby W., Albertella M., et al. Small-molecule inhibition of METTL3 as a strategy against myeloid leukaemia. Nature. 2021;593(7860):597–601. - PMC - PubMed
    1. Wang Y., Xiao Y., Dong S., et al. Antibody-free enzyme-assisted chemical approach for detection of N(6)-methyladenosine. Nat. Chem. Biol. 2020;16(8):896–903. - PubMed
    1. Mahdavi-Amiri Y., Chung Kim Chung K., Hili R. Single-nucleotide resolution of N (6)-adenine methylation sites in DNA and RNA by nitrite sequencing. Chem. Sci. 2020;12(2):606–612. - PMC - PubMed
    1. Werner S., Galliot A., Pichot F., et al. NOseq: amplicon sequencing evaluation method for RNA m6A sites after chemical deamination. Nucleic Acids Res. 2021;49(4):e23. - PMC - PubMed

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