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. 2024 Aug 22;9(3):236-242.
doi: 10.14744/hnhj.2024.47450.

Effect of Disinfection Protocols on Bacterial Reduction in Mandibular Molars

Helder Fernandes de Oliveira  1 , Luan Carlos Gomes Teixeira  1 , Ismael Fernandes Da Silva Júnior  1 , Francisco Ferreira Da Cruz  1 , Hellen Cristina Silva  1 , Alex Da Rocha Gonçalves  1 , Daniel De Almeida Decurcio  2 , Orlando Aguirre Guedes  1
Affiliations

Effect of Disinfection Protocols on Bacterial Reduction in Mandibular Molars

Helder Fernandes de Oliveira et al. Eur Endod J. .

Abstract

Objective: Evaluate the bacterial reduction effect of disinfection protocols used in lower molars infected by Enterococcus faecalis.

Methods: Eighty extracted lower molars were prepared and inoculated with E. faecalis for 2 months. The teeth were then distributed into 2 control groups (n = 4) and 4 experimental groups (n = 18) in function of the disinfection protocol utilized: G1. WaveOne Gold (WOG), passive ultrasonic irrigation (PUI) and Ultracal; G2. WOG, PUI and calcium hydroxide (CH); G3. WOG, XP-Endo Finisher (XPF) and Ultracal; G4. WOG, XPF and CH. Bacteriological samples were collected previously (S1), after preparation (S2), final agitation (S3) and intracanal dressing (S4). Microbial growth was assessed according to culture turbidity and UV spectrophotometry. Statistical analyses used the Friedman test for paired samples and Kruskal-Wallis test for non-paired data (p<0.05).

Results: No protocol eliminated E. faecalis effectively. The S2, S3 and S4 samples were statistically different from the S1 samples in G1, G2, G3 and G4 (p<0.05). Statistical differences were observed in bacterial reduction between G1 and G2 and G1 and G3 after the intracanal medicament (S4) (p<0.05).

Conclusion: The WOG + PUI + CH disinfection protocol showed higher percentages of bacterial reduction.

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Conflict of interest statement

All authors declared no conflict of interest.

Figures

Figure 1
Figure 1
Biofilm formation: (a) the specimens received a layer of epoxy resin; (b) specimen immersed in NaOCl for 30 min; (c) The set was then rinsed with distilled water and (d) coupled to a 20 mL tube with a cap containing 10 mL of BHI; (e) inoculations performed every 3 days. Platform adapted from previous studies (4, 6) NaOCl: Sodium hypochlorite, BHI: Brain Heart Infusion
Figure 2
Figure 2
Distribution of test groups according to disinfection protocol, including preparation, final agitation technique and type of intracanal medicament WOG: WaveONe Gold; PUI: Passive ultrasonic irrigation; CH: Calcium hydroxide; XPF: XP-Endo-Finisher
See this image and copyright information in PMC

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