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. 2024 Jun 27;24(1):738.
doi: 10.1186/s12903-024-04505-1.

Investigation of adhesion status of Candida species to the surface of resin materials produced at different angles with additive manufacturing

Omer F Turanoglu  1 , Esra Talay Cevlik  2 , Caner Vural  3
Affiliations

Investigation of adhesion status of Candida species to the surface of resin materials produced at different angles with additive manufacturing

Omer F Turanoglu et al. BMC Oral Health. .

Abstract

Background: The aim of this study was to evaluate the adhesion of Candida glabrata, Candida albicans, Candida krusei, Candida parapsilosis and Candida tropicalis yeasts to disk-shaped resin materials produced from resin which used in the production of surgical guide with 0, 45 and 90-degrees printing orientations by Liquid Crystal Display additive manufacturing technology.

Methods: Disk-shaped specimens were printed with surgical guide resin using the Liquid Crystal Display production technique in 3 printing orientations (0, 45 and 90-degrees). Surface roughness and contact angle values were evaluated. Real-Time PCR analysis was performed to evaluate Candida adhesion (C. glabrata, C. albicans, C. krusei, C. parapsilosis and C. tropicalis) Field emission scanning electron microscope (FESEM) images of the materials were obtained.

Results: Specimens oriented at 45-degrees demonstrated higher surface roughness (P < .05) and lower contact angle values than other groups. No significant difference was found in the adhesion of C. glabrata, C. albicans, and C. parapsilosis among specimens printed at 0, 45, and 90-degrees orientations (P > .05). A higher proportion of C. krusei and C. tropicalis was found in the specimens printed at orientation degrees of 45 = 90 < 0 with statistical significance. Analyzing the adhesion of all Candida species reveals no statistical disparity among the printing orientations.

Conclusions: The surface roughness, contact angle, and adhesion of certain Candida species are affected by printing orientations. Hence, careful consideration of the printing orientation is crucial for fabricating products with desirable properties. In 45-degree production, roughness increases due to the layered production forming steps, whereas in 0-degree production, certain Candida species exhibit high adhesion due to the formation of porous structures. Consequently, considering these factors, it is advisable to opt for production at 90-degrees, while also considering other anticipated characteristics.

Keywords: Candida; 3D printing; Microbial adhesion; Surgical guide.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Column graph derived from Real-Time PCR findings
Fig. 2
Fig. 2
Total amount of Candida adhering to materials fabricated at different printing orientations
Fig. 3
Fig. 3
Contact angle images at different printing orientations (a) at 0-degree (b) at 45-degree (c) at 90-degree
Fig. 4
Fig. 4
FESEM images of the material fabricated at 0, 45 and 90-degree orientation. (a) Control group at 200X (b) Vortexed material at 2000X (c) Vortexed material after adhesion at 2000X
See this image and copyright information in PMC

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