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. 1985 Aug;31(8):1331-4.

Specific immunoassay of alpha-amylase isoenzymes in human serum

M Gerber, K Naujoks, H Lenz, W Gerhardt, K Wulff
  • PMID: 3893797

Specific immunoassay of alpha-amylase isoenzymes in human serum

M Gerber et al. Clin Chem. 1985 Aug.

Abstract

A monoclonal antibody (66C7) was prepared that specifically binds human salivary amylase (EC 3.2.1.1); it cross reacts with human pancreatic amylase by less than 1%. Two procedures are described for determination of isoamylases in human serum with this antibody: an enzyme immunoassay for determining amylase of salivary origin, and a routine method in which this amylase is immunoprecipitated and the remaining (pancreatic) amylase activity is assayed. Results by the two methods correlate well.

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