doi: 10.1016/0092-8674(79)90351-9.
Splicing of yeast tRNA precursors: structure of the reaction intermediates
- PMID: 389432
- DOI: 10.1016/0092-8674(79)90351-9
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Splicing of yeast tRNA precursors: structure of the reaction intermediates
Cell.
1979 Sep.
Abstract
The intermediates of the yeast tRNA splicing reaction have been characterized. The intervening sequence is excised as an unique linear molecule. It has 5'-hydroxyl and 3'-phosphate termini. Correspondingly, the half-tRNA molecules are shown to have a 3'-phosphate terminus on the 5' half and 5'-hydroxyl terminus on the 3' half. These isolated halves have been shown to be active in the ligation step of tRNA splicing. Removal of the 3'-phosphate from the 5' half eliminates the ability of the 5' half to participate in ligation.
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