Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Jun 30;27(2):101-109.
doi: 10.3831/KPI.2024.27.2.101.

Pandanus amaryllifoius Roxb. Leaves Ethanol Extract Ameliorates Lipid and Proinflammatory Cytokines Profiles in a Rat Model of Dyslipidemia

Martohap Parotua Lumbanraja  1 , Kusnandar Anggadiredja  1 , Neng Fisheri Kurniati  1 , Hubbi Nashrullah Muhammad  1
Affiliations

Pandanus amaryllifoius Roxb. Leaves Ethanol Extract Ameliorates Lipid and Proinflammatory Cytokines Profiles in a Rat Model of Dyslipidemia

Martohap Parotua Lumbanraja et al. J Pharmacopuncture. .

Abstract

Objectives: Dyslipidemia has currently become a major health challenge that still opens for safer and more effective modes of treatment. The plant Pandanus amaryllifolius Roxb. (pandan) has been indicated to contain active ingredients that interfere with the pathological pathway of dyslipidemia. The aim of the study was to test the effects of pandan leaves ethanol extract on lipid and proinflammatory profiles in a rat dyslipidemic model.

Methods: Dyslipidemia was induced by administration of high-fat feed for 8 weeks. Treatments (vehicle, the reference drug simvastatin at 1.8 mg/kg, and extract at 200, 300 or 600 mg/kg) were given for 4 weeks following the completion of induction.

Results: Significant post-treatment decreases in total cholesterol, low density lipoprotein (LDL), and triglyceride levels in groups receiving all doses of extract and simvastatin were observed. Similar results were also found in regards to proinflammatory cytokines levels. Pandan extracts significantly lowered the concentrations of IL-6, TNF-α, and NFκB p65. Characterization of metabolite contents of the extract confirmed the presence of the previously suggested active alkaloids pandamarilactonine-A and B.

Conclusion: Taken together, results of the present study implied the ameliorating effects of pandan leaves ethanol extract in dyslipidemic condition which is potential for opening an avenue in combating this essential component of metabolic disorder.

Keywords: Pandanus amaryllifoius Roxb; dyslipidemia; ethanol extract; leaves; pandan; rats.

PubMed Disclaimer

Conflict of interest statement

CONFLICTS OF INTEREST The authors declare no conflicts of interest in this work.

Figures

Figure 1
Figure 1
LCMS chromatogram of pandan leaves extract. (A) Base peak chromatogram of blank and pandan leaves extract, with the suggested antidyslipidemic alkaloids pandamarilactonine-A or B (arrows); (B) Mass chromatogram of pandamarilactonine A or B.
Figure 2
Figure 2
The effects of pandan leaves extract (PAE) on lipid parameters (total cholesterol, A; LDL, B; triglyceride, C) in a rat dyslipidemic model. The dyslipidemia was induced by administering high-fat feed for 8 weeks. Upon completion of the induction, the treatments were given for 4 weeks. Gray and black bars indicate pre- and post-treatment levels, respectively. Data represents average ± SEM of 3-7 rats. *, **, ***p < 0.05, 0.01 and 0.001 vs pre-treatment, paired t-test; ##, ###p < 0.01, 0.001 vs normal, unpaired t-test.
Figure 3
Figure 3
The effects of pandan leaves extract (PAE) on proinflammatory cytokines (IL-6, A; TNFalfa, B; NFκβ p65, C) in a rat dyslipidemic model. The dyslipidemia was induced by administering high-fat feed for 8 weeks. Upon completion of the induction, the treatments were given for 4 weeks. Data represents average ± SEM of 3-4 rats. *, **, ***p < 0.05, 0.01 and 0.001 vs vehicle, ANOVA post hoc PLSD.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Kwon SH, Myong JP, Kim HA, Kim KY. Association between morbidity of non-communicable disease and employment status: a comparison between Korea and the United States. BMC Public Health. 2020;20(1):763. doi: 10.1186/s12889-020-08883-3. - DOI - PMC - PubMed
    1. Cavalcanti AM, Kusma SZ, Chomatas ERDV, Ignácio SA, Mendes EV, Moysés ST, et al. Noncommunicable diseases and their common risk factors in Curitiba, Brazil: results of a cross-sectional, population-based study. Rev Panam Salud Publica. 2018;42:e57. doi: 10.26633/RPSP.2018.57. - DOI - PMC - PubMed
    1. Crovetto M, Sepúlveda MJ. Relationship between dietary energy intake, nutritional status and cardiovascular risk in adults from the communes of Quellón and Chonchi, Chiloé, Chile. J Prev Med Hyg. 2022;63(3):E435–41. - PMC - PubMed
    1. Pirillo A, Casula M, Olmastroni E, Norata GD, Catapano AL. Global epidemiology of dyslipidaemias. Nat Rev Cardiol. 2021;18(10):689–700. doi: 10.1038/s41569-021-00541-4. - DOI - PubMed
    1. Poropat Flerin T, Božič Mijovski M, Jug B. Association between lipoprotein subfractions, hemostatic potentials, and coronary atherosclerosis. Dis Markers. 2022;2022:2993309. doi: 10.1155/2022/2993309. - DOI - PMC - PubMed

LinkOut - more resources