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. 2024:2833:145-152.
doi: 10.1007/978-1-0716-3981-8_14.

Quantifying Viable M. tuberculosis Safely Obviating the Need for High Containment Facilities

Bariki Mtafya  1 , Emmanuel Musisi  2   3 , Paschal Qwaray  1 , Emanuel Sichone  1 , Natasha Walbaum  4 , Nyanda Elias Ntinginya  1 , Stephen H Gillespie  4 , Wilber Sabiiti  5
Affiliations

Quantifying Viable M. tuberculosis Safely Obviating the Need for High Containment Facilities

Bariki Mtafya et al. Methods Mol Biol. 2024.

Abstract

Mycobacterium tuberculosis is an infectious pathogen that requires biosafety level-3 laboratory for handling. The risk of transmission is high to laboratory staff, and to manage the organism safely, it is necessary to construct high containment laboratory facilities at great expense. This limits the application of tuberculosis diagnostics to areas where there is insufficient capital to invest in laboratory infrastructure. In this method, we describe a process of inactivating sputum samples by either heat or guanidine thiocyanate (GTC) that renders them safe without affecting the quantification of viable bacteria. This method eliminates the need for level 3 containment laboratory for the tuberculosis molecular bacterial load assay (TB-MBLA) and is applicable in low- and middle-income countries.

Keywords: Accessibility; Containment level III; Diagnosis; GTC; Heat inactivation; TB-MBLA; Tuberculosis.

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References

    1. Lange C, Aarnoutse R, Chesov D, van Crevel R, Gillespie SH, Grobbel HP et al (2020) Perspective for precision medicine for tuberculosis. Front Immunol 11:566608
    1. Siddiqi SH, Rüsch-Gerdes S (2006) MGIT Procedure Manual: For BACTEC™ MGIT 960™ TB System. 2006;(July):1–52
    1. World Health Organization (2021) Technical Report on critical concentrations for drug susceptibility testing of isoniazid and the rifamycins (rifampicin, rifabutin and rifapentine), 1–86 p
    1. World Health Organisation (2022) Global tuberculosis report. Geneva
    1. Joshi R, Reingold AL, Menzies D, Pai M (2006) Tuberculosis among health-care workers in low- and middle-income countries: a systematic review. PLoS Med 3(12):2376–2391 - DOI