Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1985 Jul;82(14):4740-4.
doi: 10.1073/pnas.82.14.4740.

On ras gene function in yeast

D G Fraenkel
Comparative Study

On ras gene function in yeast

D G Fraenkel. Proc Natl Acad Sci U S A. 1985 Jul.

Abstract

Saccharomyces cerevisiae contains two RAS genes, RAS1 and RAS2. An insertion mutation in RAS2 (ras2::LEU2) does not affect growth on glucose based media but it does prevent growth on media with pyruvate or other noncarbohydrate carbon sources. This defect is pH sensitive and is most severe at pH 7 and above. The ras2::LEU2 mutation also causes markedly higher levels of glycogen in the derepressed phase of growth after glucose exhaustion. Selection for restoration of growth on pyruvate yields unlinked suppressor mutations. Some of the suppressors also reduce glycogen as well as trehalose (the other reserve carbohydrate in yeast) to levels much lower than those of wild-type strains. These suppressor mutations do not suppress the lethality of ras1 ras2 double mutants. The results indirectly accord with yeast RAS2 governing a G protein activity of adenylate cyclase.

PubMed Disclaimer

References

    1. Cell. 1984 Jun;37(2):437-45 - PubMed
    1. Cell. 1984 Mar;36(3):607-12 - PubMed
    1. Nature. 1984 Jun 7-13;309(5968):523-7 - PubMed
    1. Arch Microbiol. 1984 May;138(1):64-7 - PubMed
    1. Biochem J. 1984 Apr 15;219(2):511-8 - PubMed

Publication types