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. 2024 Jul 2;19(7):e0306142.
doi: 10.1371/journal.pone.0306142. eCollection 2024.

Iron gallic acid biomimetic nanoparticles for targeted magnetic resonance imaging

Yan Chen  1   2 , Zhaohui Zhang  1   2   3 , Zhijian Chen  4   5 , Shiqing Jiang  6 , Aikebaier Reheman  1   2 , Yifan Ouyang  1   2 , Bo Yu  4   5 , Qiuyan Chen  4   5 , Dingtai Wei  4   5
Affiliations

Iron gallic acid biomimetic nanoparticles for targeted magnetic resonance imaging

Yan Chen et al. PLoS One. .

Abstract

Developing T1-weighted magnetic resonance imaging (MRI) contrast agents with enhanced biocompatibility and targeting capabilities is crucial owing to concerns over current agents' potential toxicity and suboptimal performance. Drawing inspiration from "biomimetic camouflage," we isolated cell membranes (CMs) from human glioblastoma (T98G) cell lines via the extrusion method to facilitate homotypic glioma targeting. At an 8:1 mass ratio of ferric chloride hexahydrate to gallic acid (GA), the resulting iron (Fe)-GA nanoparticles (NPs) proved effective as a T1-weighted MRI contrast agent. T98G CM-coated Fe-GA NPs demonstrated improved homotypic glioma targeting, validated through Prussian blue staining and in vitro MRI. This biomimetic camouflage strategy holds promise for the development of targeted theranostic agents in a safe and effective manner.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Scheme 1
Scheme 1. Schematic illustration for the synthesis of CM-Fe-GA NPs.
Fig 1
Fig 1
AFM images of Fe-GA NPs (a) and vertical distance of the nanoparticle measured from AFM images by software Nanoscope Analysis (b). The zeta potential of different groups (c), and DLS photograph of T98G CM-Fe-GA NPs (d).
Fig 2
Fig 2
The relaxation rates (R1) versus different concentrations of iron (a) and T1 MR images (b) of Fe-GA NPs at different mass ratio of FeCl3·6H2O to GA.
Fig 3
Fig 3. Specific binding of targeted nanoprobe in vitro for T98G cell.
On Prussian blue stain, (a) T98G CM-Fe-GA NPs shows strong binding to cell surface of T98G cells by comparison with (b) Fe-GA NPs and (c) control. (d) Quantified mean intensities of the blue color by the software Image J. The error bars indicate the s.d. (n = 5, ***P<0.001 from an analysis of variance with two-tailed t test).
Fig 4
Fig 4
The photographs (A), T1-weighted MR images (B), and T1 relaxation time (C) of T98G cells incubated with T98G CM-Fe-GA NPs (a), Fe-GA NPs (b) and control group (c). The error bars indicate the s.d. (n = 3, ***P<0.001 from an analysis of variance with two-tailed t test).
See this image and copyright information in PMC

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