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. 2024 Sep:532:113717.
doi: 10.1016/j.jim.2024.113717. Epub 2024 Jul 2.

Belimumab concentration measurements using a homologous anti-idiotype immunoassay

Floris C Loeff  1 , Ioannis Parodis  2 , Tomas Walhelm  3 , Andreas Jönsen  4 , Dionysis Nikolopoulos  5 , Christopher Sjöwall  3 , Anders A Bengtsson  4 , Dorien Kos  6 , Astrid van Leeuwen  1 , Bryan van den Broek  1 , Lisanne Dijk  1 , Jorn Jeremiasse  1 , Birgit S Blomjous  7 , Alexandre E Voskuyl  7 , Gerrit Jan Wolbink  8 , Irene E M Bultink  7 , Theo Rispens  9
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Free article

Belimumab concentration measurements using a homologous anti-idiotype immunoassay

Floris C Loeff et al. J Immunol Methods. 2024 Sep.
Free article

Abstract

Monitoring belimumab concentrations in patients can be a valuable tool for assessing treatment response and for personalizing drug doses. Various assay formats may be used to measure concentrations of therapeutic monoclonal antibodies. A particularly useful format involves the use of anti-idiotype monoclonal antibodies, selected to be highly specific to the antibody of interest. Here, we describe the development of a specific, high-affinity anti-idiotype antibody to belimumab, and the application of this antibody in a homologous sandwich ELISA to measure belimumab concentrations.

Keywords: Anti-idiotype antibodies; Belimumab; PK/ADA assays; Rabbit monoclonal antibodies.

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