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. 2024 Mar 6:54:110296.
doi: 10.1016/j.dib.2024.110296. eCollection 2024 Jun.

Whole genome sequence data of the multidrug-resistant Proteus mirabilis-MN029, a model bacterial strain for the evaluation of antibacterial activity from selected native plant extracts

Katlego P P Makale  1 , Motlatsi Nketsang  1 , Gaolathe Rantong  1 , Abdullah Makhzoum  1 , Teddie O Rahube  1
Affiliations

Whole genome sequence data of the multidrug-resistant Proteus mirabilis-MN029, a model bacterial strain for the evaluation of antibacterial activity from selected native plant extracts

Katlego P P Makale et al. Data Brief. .

Abstract

Antimicrobial resistance remains a significant global and One Health threat, owing to the diminishing effectiveness of antibiotics against rapidly evolving multidrug-resistant bacteria, and the limited innovative research towards the development of new antibiotic therapeutics. In this article, we present the whole-genome sequence data of Proteus mirabilis-MN029 obtained from highly accurate long-read PacBioⓇ HiFi technology. The antibacterial activities of the selected African native plant species were also evaluated using the disk diffusion method. Acquired antibiotic resistance genes and chromosomal mutations corresponding to antibiotics of clinical importance were identified from genomic data. Using ethlyl acetate as solvent, Pterocarpus angolensis leaf extracts showed the most promising antibacterial effects against Proteus mirabilis-MN029. These datasets will be useful for future experimental research aimed at designing new antibacterial drugs from plant extracts that are effective alone or in combination with existing antibiotics to overcome multidrug-resistance mechanisms.

Keywords: African plant extracts; Antimicrobial properties; Antimicrobial resistance; Long read sequencing; One Health.

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Figures

Fig 1
Fig. 1
Genome analysis of P. mirabilis-MN029. (A) Circular graphical display of the distribution of the genome annotations; Outer to inner rings correspond to the chromosome, Coding sequences (CDS) on the forward strand (CDS-Fwd), CDS on the reverse strand (CDS-Rev), Non-coding sequences (Non-CDS), CDS with homology to known antimicrobial resistance (AMR) genes, virulence factors (VF), transporters and drug targets. (B). Identification and phylogenetic analysis of the MDR bacterial strain.
Fig 2
Fig. 2
Zones of inhibition shown by different crude extracts at different concentrations against P. mirabilis-MN029. Disk diffusion was performed in triplicate, and the error bars represent the standard error of the mean (SEM). ∗ P < 0.05 compared with the control, ∗∗ P < 0.005 in comparison to control, ∗∗∗ P < 0.001 in comparison to the positive control, ∗∗∗∗ P < 0.0001 in comparison to the control and ns means there is no significant difference in comparison to the control. Keys: dH2O; distilled water, PC is positive control (kanamycin, 30 µg).
See this image and copyright information in PMC

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