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. 1985 Jul 31;130(2):692-9.
doi: 10.1016/0006-291x(85)90472-3.

Purification and characterization of recombinant human interleukin-2 produced in Escherichia coli

K Kato, T Yamada, K Kawahara, H Onda, T Asano, H Sugino, A Kakinuma

Purification and characterization of recombinant human interleukin-2 produced in Escherichia coli

K Kato et al. Biochem Biophys Res Commun. .

Abstract

Recombinant human interleukin-2 (rIL-2) produced in Escherichia coli was purified to apparent homogeneity by cation exchange chromatography and reverse phase high performance liquid chromatography. The amino acid composition, amino terminal amino acid sequence, and carboxyl terminal amino acid were consistent with those deduced from the cDNA sequence. Besides the molecular species with the amino terminal Ala, the purified preparation contained another species having an additional Met residue at the amino terminus corresponding to the initiation codon AUG. The molar absorption coefficient of rIL-2 was determined to be 9.58 X 10(3) M-1 cm-1 at 280nm in water. Ultracentrifugal analyses revealed that it existed as a monomeric form in 0.1 M NaCl. The apparent sedimentation coefficient (S20,w) was calculated to be 1.8 S.

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