Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Jul 5;39(1):171.
doi: 10.1007/s10103-024-04116-w.

Effect of combined red and infrared wavelengths on inflammation, hemorrhage, and muscle damage caused by Bothrops leucurus snake venom

Gisele Dias da Silva  1 , Fabiana Lessa Silva  2 , Anaiá da Paixão Sevá  3 , Danilo Machado Deorce  4 , Nerildo de Jesus da Costa Junior  4 , Fernanda Amaral Silva  4 , Fernando Alzamora Filho  3
Affiliations

Effect of combined red and infrared wavelengths on inflammation, hemorrhage, and muscle damage caused by Bothrops leucurus snake venom

Gisele Dias da Silva et al. Lasers Med Sci. .

Abstract

To evaluate the effects of red and infrared wavelengths, separately and combined, on the inflammatory process and collagen deposition in muscle damage caused by B. leucurus venom. 112 mice were inoculated with diluted venom (0.6mg/kg) in the gastrocnemius muscle. The animals were divided into four groups: one control (CG) and three treatments, namely: 1) red laser (λ=660 nm) (RG), 2) infrared laser (λ=808 nm) (IG) and 3) red laser (λ=660 nm) + infrared (λ=808 nm) (RIG). Each group was subdivided into four subgroups, according to the duration of treatment application (applications every 24 hours over evaluation times of up to 144 hours). A diode laser was used (0.1 W, CW, 1J/point, ED: 10 J/cm2). Both wavelengths reduced the intensity of inflammation and the combination between them significantly intensified the anti-inflammatory response. Photobiomodulation also changed the type of inflammatory infiltrate observed and RIG had the highest percentage of mononuclear cells in relation to the other groups. Hemorrhage intensity was significantly lower in treated animals and RIG had the highest number of individuals in which this variable was classified as mild. As for collagen deposition, there was a significant increase in RG in relation to CG, in RIG in relation to CG and in RIG in relation to IG. Photobiomodulation proved to be effective in the treatment of inflammation and hemorrhage caused by B. leucurus venom and stimulated collagen deposition. Better results were obtained with the combined wavelengths.

Keywords: Inflammatory infiltrate; Photobiomodulation; Skeletal muscle; Tissue repair.

PubMed Disclaimer

References

    1. Mamede CCN, de Sousa Simamoto BB, da Cunha Pereira DF, de Oliveira Costa J, Ribeiro MSM et al (2020) Edema, hyperalgesia and myonecrosis induced by Brazilian bothropic venoms: overview of the last decade. Toxicon 187:10–18. https://doi.org/10.1016/j.toxicon.2020.08.016 - DOI - PubMed
    1. Gutiérrez JM, Calvete JJ, Habib AG, Harrison RA, Williams DJ et al (2017) Snakebite envenoming. Nat Rev Dis Primers 3(1):17063. https://doi.org/10.1038/nrdp.2017.63 - DOI - PubMed
    1. Hamblin MR (2017) Mechanisms and applications of the anti-inflammatory effects of photobiomodulation. AIMS Biophys 4(3):337–361. https://doi.org/10.3934/biophy.2017.3.337 - DOI - PubMed - PMC
    1. Hernández R, Cabalceta C, Saravia-Otten P, Chaves A, Gutiérrez JM et al (2011) Poor Regenerative Outcome after Skeletal Muscle Necrosis Induced by Bothrops asper Venom: Alterations in Microvasculature and Nerves. PLoS ONE 6(5):e19834. https://doi.org/10.1371/journal.pone.0019834 - DOI - PubMed - PMC
    1. Lauria PSS, Maia-Marques R, de Oliveira R, Nunes VLC, Casais-e-Silva LL (2021) Effects of photobiomodulation therapy on the local experimental envenoming by Bothrops leucurus snake. J Photochem Photobiol B 214:112087. https://doi.org/10.1016/j.jphotobiol.2020.112087 - DOI - PubMed

LinkOut - more resources