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. 2024 Jul 5;24(1):673.
doi: 10.1186/s12879-024-09523-6.

Dental complications as a potential indicator of Redondovirus infection: a cross-sectional study

Alireza Mohebbi  1   2 , Seyed Jalal Kiani  1 , Khadijeh Khanaliha  3 , Tahereh Donyavi  4 , Nikoo Emtiazi  5 , Kimia Sharifian  1 , Maryam Mohebbi  1 , Amytis Gholami  1 , Farzane Behnezhad  1 , Mohammad Abbasi-Kolli  1 , Farzaneh Dehghani-Dehej  1 , Farah Bokharaei-Salim  6
Affiliations

Dental complications as a potential indicator of Redondovirus infection: a cross-sectional study

Alireza Mohebbi et al. BMC Infect Dis. .

Abstract

Background: Redondoviridae is a newly discovered virus family linked to oral and respiratory conditions in people, while there is still debate about whether it is also coinfected with other respiratory viruses. This study aimed to determine the frequency of Redondovirus (ReDoV) in nasopharyngeal samples and to investigate any possible links to SARS-CoV-2 infections.

Methods: A polymerase chain reaction (PCR) test was conducted on 731 nasopharyngeal samples from individuals referred to medical centers in Tehran, Iran, for SARS-CoV-2 testing to investigate the prevalence of ReDoV. An oral interview was performed to complete information on dental issues and the individuals' demographics, symptoms, and vaccination history.

Results: The prevalence of ReDoV was 25.99%, and 15.26% had a coinfection with SARS-CoV-2. No notable correlation was found regarding ReDoVs and SARS-CoV-2 infections (p > 0.05). Women had a higher ReDoV positivity rate of 18.47% compared to men at 7.52% (p = 0.12), and there was no significant correlation between age groups and ReDoV presence. Nonetheless, a significant association was noted between ReDoVs and dental/gum issues (p < 0.0001, OR: 13.0326). A phylogenetic analysis showed that ReDoVs originated from various human-related clusters.

Conclusions: These results highlight the potential for detecting ReDoVs in nasopharyngeal samples of people with gum or dental issues. Additionally, conducting more ReDoV epidemiological research and proposing oral health as a possible marker for ReDoV infections is important.

Keywords: Oral health; Periodontitis; Redondovirus; Respiratory infection; SARS-CoV-2.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Radial phylogram re-rooted with Mosquito VEM Anellovirus SDBVL A (NC_076121.1). The sequenced data of ReDoVs from the present study are colored. PP319426 and PP319425 are phylogenetically related to the human respiratory-associated Brisaviruses. PP319427 and PP319428 are genetically close to oral-associated Vientoviruses, and PP319424 is related to lung-associated Vientoviruses. Blue circles represent bootstraps ranging from 4 (smallest) to 100 (largest)
Fig. 2
Fig. 2
PCR amplification and gel electrophoresis of ReDoV genome. The gel electrophoresis demonstrates the presence of ReDoV DNA in the positive samples (Black arrows) with a size of 187 nt long. L100 represents the DNA marker (ladder) for size reference, while lane numbers show the PCR products of individual samples
Fig. 3
Fig. 3
Calculated PCR efficiency with a rounded slope of 3.34. 350 ng viral DNA was also detected by this PCR method
Fig. 4
Fig. 4
Association of ReDoVs genome prevalence in different age categories, sex, SARS-CoV-2 results, and gum or dental complications results. The Red arrows highlight the groups in which negative or positive results of ReDoV are significant based on Fisher’s exact test
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