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. 1985 Sep;163(3):1267-9.
doi: 10.1128/jb.163.3.1267-1269.1985.

Single transporter for sulfate, selenate, and selenite in Escherichia coli K-12

C Lindblow-Kull, F J Kull, A Shrift

Single transporter for sulfate, selenate, and selenite in Escherichia coli K-12

C Lindblow-Kull et al. J Bacteriol. 1985 Sep.

Abstract

A Michaelis-Menten kinetic analysis of the transport of sulfate, selenate, and selenite into Escherichia coli K-12 showed that the three dianions were transported by the same carrier. Km values, used as a measure of the affinity of each ligand for the carrier, showed that sulfate was bound 5 times more tightly than selenate and 37 times more tightly than selenite. The specificity ratio, Vmax/Km, also indicated that sulfate was the preferred ligand. There was little difference in the ratios for selenate and selenite.

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