Comparative Study
doi: 10.1016/s0092-1157(85)80009-3.
A rapid quantitative microtest for live, attenuated rabies vaccine
- PMID: 3897234
- DOI: 10.1016/s0092-1157(85)80009-3
Item in Clipboard
Comparative Study
A rapid quantitative microtest for live, attenuated rabies vaccine
J Biol Stand.
1985 Jul.
Abstract
A rapid fluorescent antigen test (RFAT) was developed in microplates to evaluate quantitatively attenuated, live rabies vaccine, ERA strain, at different stages of vaccine manufacture. This technique is based on the principle of the rapid fluorescent focus inhibition test (RFFIT). Several samples of bulk rabies vaccine (ERA strain) were tested in parallel using the RFAT and the mouse lethal dose 50 (M-LD50) test, the method currently used to evaluate vaccine potency. Although the two tests were found to be comparable in sensitivity the RFAT is of significant benefit in terms of simplicity and rapidity for potency evaluation during vaccine manufacture.
Similar articles
-
In vitro rabies vaccine potency appraisal by ELISA: advantages of the immunocapture method with a neutralizing anti-glycoprotein monoclonal antibody.Biologicals. 1990 Oct;18(4):321-30. doi: 10.1016/1045-1056(90)90037-z. Biologicals. 1990. PMID: 2285504
-
Evaluation and Correlation of Rabies Vaccine Potency Using the National Institute of Health, Rapid Focus Fluorescent Inhibition, and Passive Hemagglutination Tests.Viral Immunol. 2022 Mar;35(2):159-169. doi: 10.1089/vim.2021.0181. Epub 2022 Feb 1. Viral Immunol. 2022. PMID: 35104162
-
Detection of rabies virus antigen or antibody using flow cytometry.Cytometry B Clin Cytom. 2006 Sep 15;70(5):335-43. doi: 10.1002/cyto.b.20104. Cytometry B Clin Cytom. 2006. PMID: 16739219
-
Generation and characterization of a genetically modified live rabies vaccine strain with attenuating mutations in multiple viral proteins and evaluation of its potency in dogs.Vaccine. 2023 Jul 25;41(33):4907-4917. doi: 10.1016/j.vaccine.2023.06.076. Epub 2023 Jul 1. Vaccine. 2023. PMID: 37400284
-
New approaches to the development of live attenuated rabies vaccines.Hybrid Hybridomics. 2002 Apr;21(2):129-34. doi: 10.1089/153685902317401735. Hybrid Hybridomics. 2002. PMID: 12031103 Review.
Cited by
-
Safety and immunogenicity of ERA strain of rabies virus propagated in a BHK-21 cell line.Can J Vet Res. 1989 Oct;53(4):438-44. Can J Vet Res. 1989. PMID: 2686831 Free PMC article.
-
Safety and immunogenicity of a vaccine bait containing ERA strain of attenuated rabies virus.Can J Vet Res. 1987 Oct;51(4):460-4. Can J Vet Res. 1987. PMID: 3330965 Free PMC article.
-
Stability of attenuated live virus rabies vaccine in baits targeted to wild foxes under operational conditions.Can Vet J. 2001 May;42(5):368-74. Can Vet J. 2001. PMID: 11360859 Free PMC article.
-
Duration of immunity in foxes vaccinated orally with ERA vaccine in a bait.Can J Vet Res. 1997 Jan;61(1):39-42. Can J Vet Res. 1997. PMID: 9008799 Free PMC article.
-
Studies on efficacy and stability of a vaccine bait containing ERA strain of rabies virus propagated in a BHK-21 cell line.Can J Vet Res. 1992 Apr;56(2):135-41. Can J Vet Res. 1992. PMID: 1591656 Free PMC article.