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. 2024 Jun 7;16(6):e61896.
doi: 10.7759/cureus.61896. eCollection 2024 Jun.

Effects of Multivitamin-Mineral Supplementation on Chronic Stress-Induced Oxidative Damage in Swiss Albino Mice

Nida Suhail  1 , Tehreem Aftab  2 , Anwar Alruwaili  1 , Daliyah Alruwaili  1
Affiliations

Effects of Multivitamin-Mineral Supplementation on Chronic Stress-Induced Oxidative Damage in Swiss Albino Mice

Nida Suhail et al. Cureus. .

Abstract

Objective: Stress is a hazardous occurrence that causes a variety of physiological and behavioral responses in a person. It increases energy metabolism and enhances oxidative stress, both of which are implicated in the pathophysiology of several diseases. Numerous vitamins and minerals have the ability to modulate oxidative stress. The present investigation aimed to evaluate the effectiveness of a multivitamin-mineral (MM) supplement in addressing oxidative imbalances caused by chronic stress in the plasma, hepatic, and renal tissues of Swiss albino mice.

Methods: Thirty healthy male Swiss albino mice were randomly assigned to one of the three groups, with 10 animals each: control, unpredictable chronic stress (UCS), and MM + UCS. The experiment lasted for four weeks, after which all the animals underwent cervical decapitation, and samples of their blood, liver, and kidney were taken for biochemical studies. DNA damage analysis was performed on lymphocytes.

Results: Exposure to UCS negatively affected all biochemical markers, as indicated by reduced levels of antioxidants (superoxide dismutase, catalase, glutathione S-transferase, glutathione reductase, and reduced glutathione) in the plasma, liver, and kidney tissues, along with enhanced levels of lipid peroxidation and marker enzymes. MM supplementation normalized the deranged biochemical markers in stress-exposed mice. The results of DNA damage supported the biochemical findings mentioned above.

Conclusion: The findings suggest that MM supplementation could help reduce oxidative disturbances caused by stress in both healthy and diseased conditions.

Keywords: antioxidants; multivitamin-mineral; oxidative damage; swiss albino mice; unpredictable chronic stress.

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Conflict of interest statement

Human subjects: All authors have confirmed that this study did not involve human participants or tissue. Animal subjects: Institutional Animal Ethics Committee and Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA) Issued protocol number 714/02/a/CPCSEA. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work.

Figures

Figure 1
Figure 1. MDA levels in circulation, liver, and kidney of mice on UCS and MM + UCS treatments
Data represent mean ± SEM of 10 mice in each group ap<0.001 when compared to the control group bp<0.001 when compared to the UCS group MDA: malondialdehyde, UCS: unpredictable chronic stress, MM: multivitamin-mineral
Figure 2
Figure 2. Alterations in the levels of GSH in the circulation, liver, and kidney of mice on UCS and MM + UCS treatments
Data represent mean ± SEM of 10 mice in each group ap<0.001 when compared to the control group bp<0.001 when compared to the UCS group GSH: reduced glutathione, UCS: unpredictable chronic stress, MM: multivitamin-mineral
Figure 3
Figure 3. Single-cell gel electrophoresis of mice lymphocytes showing comets (100×). (A) Control, (B) UCS, and (C) MM + UCS
UCS: unpredictable chronic stress, MM: multivitamin-mineral
See this image and copyright information in PMC

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