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. 2024 Jun 24:15:1289653.
doi: 10.3389/fendo.2024.1289653. eCollection 2024.

Possible involvement of sialidase and sialyltransferase activities in a stage-dependent recycling of sialic acid in some organs of type 1 and type 2 diabetic rats

Osas Graham Erhabor  1 , Peter Obochi  1 , Murtala Bindawa Isah  2 , Mohammed Aliyu Usman  1 , Ismaila Alhaji Umar  1 , Mthokozisi B C Simelane  3 , Mohammed Nasir Shuaibu  1 , Md Shahidul Islam  4 , Mohammed Auwal Ibrahim  1
Affiliations

Possible involvement of sialidase and sialyltransferase activities in a stage-dependent recycling of sialic acid in some organs of type 1 and type 2 diabetic rats

Osas Graham Erhabor et al. Front Endocrinol (Lausanne). .

Abstract

Background: Type 1 (T1D) and type 2 (T2D) diabetes lead to an aberrant metabolism of sialoglycoconjugates and elevated free serum sialic acid (FSSA) level. The present study evaluated sialidase and sialyltranferase activities in serum and some organs relevant to diabetes at early and late stages of T1D and T2D.

Methods: Sialic acid level with sialidase and sialyltransferase activities were monitored in the serum, liver, pancreas, skeletal muscle and kidney of diabetic animals at early and late stages of the diseases.

Results: The FSSA and activity of sialidase in the serum were significantly increased at late stage of both T1D and T2D while sialic acid level in the liver was significantly decreased in the early and late stages of T1D and T2D, respectively. Furthermore, the activity of sialidase was significantly elevated in most of the diabetes-relevant organs while the activity of sialyltransferase remained largely unchanged. A multiple regression analysis revealed the contribution of the liver to the FSSA while pancreas and kidney contributed to the activity of sialidase in the serum.

Conclusions: We concluded that the release of hepatic sialic acid in addition to pancreatic and renal sialidase might (in)directly contribute to the increased FSSA during both types of diabetes mellitus.

Keywords: sialic acid; sialidase; sialoconjugates; sialyltransferase; type 1 diabetes; type 2 diabetes.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
The weekly non-fasting blood glucose levels of type 1 and type 2 diabetic rats in relation to normal control. Data for each group are presented as mean ± standard deviation of 16 and 8 animals for 1 - 3 and 4-9 weeks intervals, respectively. NCG: Normal Control Group, T1DG: Type 1 Diabetic Group, T2DG: Type 2 Diabetic Group. Different alphabets within a week indicate significant difference (P < 0.05).
Figure 2
Figure 2
Sialic acid distribution with sialidase and sialyltransferase activities of liver (A), pancreas (B), skeletal muscle (C) and kidney (D) of type 1 and type 2 diabetic rats at early (3rd week) and late (9th week) stages. Data are presented as mean ± standard deviation of 8 animals. NCG: Normal Control Group, T1DG: Type 1 Diabetic Group, T2DG: Type 2 Diabetic Group. Different alphabets within a week indicate significant difference (P < 0.05).
See this image and copyright information in PMC

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