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. 2024 Oct;66(10):2079-2082.
doi: 10.1111/jipb.13741. Epub 2024 Jul 10.

Efficient and transformation-free genome editing in pepper enabled by RNA virus-mediated delivery of CRISPR/Cas9

Chenglu Zhao  1   2   3 , Huanhuan Lou  1   2   3 , Qian Liu  1   2   3 , Siqi Pei  1   2   3 , Qiansheng Liao  4 , Zhenghe Li  1   2   3
Affiliations

Efficient and transformation-free genome editing in pepper enabled by RNA virus-mediated delivery of CRISPR/Cas9

Chenglu Zhao et al. J Integr Plant Biol. 2024 Oct.

Abstract

Tomato spotted wilt virus-mediated delivery of CRISPR/Cas9 bypasses the need for stable transformation and permits efficient, DNA-free genome editing in pepper. Remarkably, up to 77.9% of regenerated pepper plants contained heritable edits. This method has been validated with two pepper varieties and is compatible with existing tissue culture protocols.

Keywords: CRISPR/Cas9; Capsicum annuum; genome editing; pepper; regeneration; tomato spotted wilt virus; transient delivery; viral vector.

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References

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