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. 2024 Jun 25;105(2):137-147.
doi: 10.5114/bta.2024.139753. eCollection 2024.

Phytochemical composition, antioxidant, antibacterial, and enzyme inhibitory activities of organic extracts from flower buds of Cleistocalyx operculatus (Roxb.) Merr. et Perry

Doan Thien Thanh  1   2 , Vo Kieu Oanh  2 , Hoang Chinh Nguyen  3 , Luong Thi My Ngan  1 , Tran Trung Hieu  1
Affiliations

Phytochemical composition, antioxidant, antibacterial, and enzyme inhibitory activities of organic extracts from flower buds of Cleistocalyx operculatus (Roxb.) Merr. et Perry

Doan Thien Thanh et al. BioTechnologia (Pozn). .

Abstract

Cleistocalyx operculatus flower buds have been widely used in traditional medicine because of their rich content of bioactive constituents. In this study, we obtained seven solvent extracts from the flower buds and evaluated their total phenolic (TPC), flavonoid (TFC), tannin (TTC), triterpenoid saponin (TSC), and alkaloid (TAC) contents. We assessed antioxidant activities using the DPPH assay and also looked at antimicrobial and enzyme inhibitory effects. The water extract possessed the highest TPC (328.9 mg GAE/g extract), followed by ethanol, methanol, and hexane extracts (85.4-101.5 mg GAE/g extract). Chloroform, butanol, ethyl acetate, and ethanol extracts had high TSCs (245.4-287.2 mg OAE/g extract). The hexane extract was richest in TTC and TFC (32.7 mg CE/g extract and 81.1 mg QE/g extract, respectively). Ethanol and methanol extracts exhibited the strongest antioxidant activities (IC50 values of 25.2 and 30.3 μg/ml, respectively), followed by the water extract (IC50 of 40.2 μg/ml). The hexane extract displayed the most growth-inhibitory activity against Helicobacter pylori ATCC51932 and ATCC43504 strains and Salmonella enterica serovar Typhimurium ATCC13311 (MIC values of 0.06, 0.13, and 0.4 mg/ml, respectively). Moreover, the hexane extract revealed the strongest inhibition of H. pylori urease activity (IC50 of 4.51 μg/ml), whereas the water and methanol extracts had potent inhibitory effects on α-glucosidase activity (IC50 values of 9.9 and 15.1 μg/ml, respectively). These flower bud extracts could be used for health protection, especially in preventing bacterial infections and inhibiting enzymes associated with various human diseases. Further investigation into the application of C. operculatus flower buds in the food and pharmaceutical industries is necessary.

Keywords: Cleistocalyx operculatus; antibacterial activity; antioxidant activity; enzyme inhibition; flower bud; phytochemical composition.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
DPPH radical scavenging IC50 values of different C. operculatus flower bud extracts; ns – depicts no significant difference at P > 0.05, * indicates a significant difference at P < 0.05, and **** indicates a significant difference at P < 0.0001 using one-way ANOVA by Tukey’s multiple comparison test
See this image and copyright information in PMC

References

    1. Amin M., Anwar F., Naz F., Mehmood T., Saari N. (2013) Anti-Helicobacter pylori and urease inhibition activities of some traditional medicinal plants. Molecules 18(2): 2135–2149. 10.3390/molecules18022135 - DOI - PMC - PubMed
    1. Bajpai V.K., Dung N.T., Suh H.J., Kang S.C. (2010) Antibacterial activity of essential oil and extracts of Cleistocalyx operculatus buds against the bacteria of Xanthomonas spp. J. Am. Oil Chem. Soc. 87: 1341–1349. 10.1007/s11746-010-1623-9 - DOI
    1. Broadhurst R.B., Jones W.T. (1978) Analysis of condensed tannins using acidified vanillin. J Sci. Food Agric. 29(9): 788–794. 10.1002/jsfa.2740290908 - DOI
    1. Dao T.T., Tung B.T., Nguyen P.H., Thuong P.T., Yoo S.S., Kim E.H., Kim S.K., Oh W.K. (2010) C-methylated flavonoids from Cleistocalyx operculatus and their inhibitory effects on novel influenza A (H1N1) neuraminidase. J. Nat. Prod. 73: 1636–1642. 10.1021/np1002753 - DOI - PubMed
    1. Du H., Li H., Wu P., Xue J., Wu Y., Wei X., Liu B. (2022) C-methyl flavonoid from the leaves of Cleistocalyx conspersipunctatus: α-glucosidase inhibitory, molecular docking simulation and biosynthetic pathway. Arab. J. Chem. 15(4): 103687. 10.1016/j.arabjc.2022.103687 - DOI

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