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. 2024 Jun;27(6):598-602.
doi: 10.4103/JCDE.JCDE_174_24. Epub 2024 Jun 6.

Evaluating the ability of cultivated odontoblasts to form dentin-like tissue in vitro using fibroblast growth factor and insulin-like growth factor

Karan Bhargava  1 , Srinidhi Surya Raghavendra  1 , Sanjyot Mulay  1 , Ajit Hindlekar  1 , Avinash Kharat  2 , Supriya Kheur  3
Affiliations

Evaluating the ability of cultivated odontoblasts to form dentin-like tissue in vitro using fibroblast growth factor and insulin-like growth factor

Karan Bhargava et al. J Conserv Dent Endod. 2024 Jun.

Abstract

Aim: The aim of the study was to evaluate the ability of cultivated odontoblast to form dentin-like tissue using fibroblast growth factor (FGF) and insulin-like growth factor (IGF).

Materials and methods: Dental pulp stem cells (DPSCs) were extracted from 10 human teeth. They were isolated and cultivated in vitro with the use of stem cell markers. The human DPSCs were characterized for trilineage differentiation. They were then differentiated into odontoblasts. The ability of cultivated odontoblasts to form dentin-like tissue was evaluated using FGF and IGF.

Results: IGF showed superior ability to form dentin-like tissue as compared to FGF. The addition of FGF showed no significant difference in the formation of dentin-like tissue. A combination of FGF and IGF in odontoblast showed an enhanced ability to form dentin-like tissue.

Conclusion: The use of growth factors IGF and FGF with dental stem cells showed a greater potential to form dentin-like tissue. This can profoundly alter the paradigms of conservative vital pulp therapy, which may eventually make it possible to treat dental diseases by regeneration of lost dentine.

Keywords: Dental pulp stem cells; dentin; fibroblast growth factor; insulin-like growth factor.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Graph 1
Graph 1
Growth curve
Figure 1
Figure 1
(A-C) Tri lineage differentiation of human dental pulp cells
Figure 2
Figure 2
Odontogenic differentiation
Graph 2
Graph 2
Cell differentiation with different growth factors
See this image and copyright information in PMC

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