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. 1985 Apr:63 ( Pt 2):147-56.
doi: 10.1038/icb.1985.16.

Optimised growth of human epidermal cells in vitro without the use of a feeder layer or collagen substrate

C H Thompson, B R Rose, Y E Cossart

Optimised growth of human epidermal cells in vitro without the use of a feeder layer or collagen substrate

C H Thompson et al. Aust J Exp Biol Med Sci. 1985 Apr.

Abstract

A culture method has been developed which enables a reasonably large quantity of human epidermal cells to be grown in vitro without the use of irradiated 3T3 feeder layers or collagen substrates. These cultures become confluent within 2 or 3 weeks of initiation, and may be sub-cultured several times over their 16-week average lifespan provided that the calcium levels in the cultured medium are controlled. Some of the potential uses of these cultures are briefly discussed.

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