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. 2024 Jul 11;21(1):156.
doi: 10.1186/s12985-024-02430-x.

Evaluation of the FPMC respiratory panel for detection of respiratory tract pathogens in nasopharyngeal swab and sputum specimens

Li Xue  1 , Jianhong Zhu  1 , Ke Lei  1 , Zeshi Liu  1 , Yiwei Tang  2 , Bo Zhong  3 , Ning Gao  1 , Chaoliang Xiong  1 , Jing Lei  1 , Ying Tian  1 , Weixiao Zhou  1 , Nan Feng  1 , Xue Zhang  1 , Dong Chen  1 , Jing Li  1 , Yan Geng  4
Affiliations

Evaluation of the FPMC respiratory panel for detection of respiratory tract pathogens in nasopharyngeal swab and sputum specimens

Li Xue et al. Virol J. .

Abstract

Objectives: The performance of the new Respiratory Pathogen panel (fluorescent probe melting curve, FPMC) for the qualitative detection of 12 organisms (chlamydia pneumoniae, mycoplasma pneumoniae, adenovirus, influenza A virus, influenza B virus, parainfluenza virus, rhinovirus, etc.) was assessed.

Methods: Prospectively collected nasopharyngeal swab (NPS) and sputum specimens (n = 635) were detected by using the FPMC panel, with the Sanger sequencing method as the comparative method.

Results: The overall percent concordance between the FPMC analysis method and the Sanger sequencing method was 100% and 99.66% for NPS and sputum specimens, respectively. The FPMC testified an overall positive percent concordance of 100% for both NPS and sputum specimens. The FPMC analysis method also testified an overall negative percent concordance of 100% and 99.38% for NPS and sputum specimens, respectively.

Conclusions: The FPMC analysis method is a stable and accurate assay for rapid, comprehensive detecting for respiratory pathogens.

Keywords: Fluorescent probe melting curve PCR; Molecular detection; Respiratory pathogen.

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Conflict of interest statement

The authors declare no competing interests.

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