First draft genome sequence data of TA4-1, the type strain of Gram-positive bacterium Streptomyces chiangmaiensis

Abstract

TA4-1 is the type strain of Streptomyces chiangmaiensis. The TA4-1 strain was isolated from a stingless bee (Tetragonilla collina). Here we present the draft genome sequence data of S. chiangmaiensis TA4-1. The Illumina NextSeq 550 sequencer was used to generate paired-end reads from the genomic DNA of the pure culture of S. chiangmaiensis TA4-1. The draft genome sequence of strain TA4-1 consists of 776 contigs with a total size of 9,707,984 base pairs, an N50 of 32,937 base pairs, and a GC content of 69.73 %. Digital DNA-DNA hybridisation (dDDH) and average nucleotide identity (ANI) analysis showed that S. yaanensis CGMCC 4.7035 had the highest dDDH value (32.7 %) and ANIm value (88.50 %) when compared with TA4-1. The presented data indicate the potential for a reference genome sequence in bacterial taxonomy, comparative genomics, and the investigation of bioactive compound biosynthesis in S. chiangmaiensis TA4-1. The draft genome sequence data have been deposited at NCBI under the Bioproject accession number PRJNA680432.

Keywords: Desferrioxamine; Flaviolin; Nonribosomal peptide; Polyketide; Terpene; Type strain.

Fig. 1

The genome map of S. chiangmaiensis TA4-1 was created using Proksee [5]. Blue arrows indicate coding DNA sequences (CDSs), and grey arrows represent contigs. The green and purple peaks represent GC skew+ and GC skew-, respectively, and the black peaks indicate GC content.

Fig. 2

The phylogenomic tree was reconstructed using whole-genome sequence data from S. chiangmaiensis TA4-1 and its closely related type strain on the TYGS platform. Branch numbers were determined based on pseudo-bootstrap support values greater than 70 % from 100 replicates using Genome Blast Distance Phylogeny (GBDP), with an average branch support of 75.0 %.