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. 2024 Jun 27:11:1355448.
doi: 10.3389/fmolb.2024.1355448. eCollection 2024.

Automated classification of group B Streptococcus into different clonal complexes using MALDI-TOF mass spectrometry

Lianfen Huang  1 , Kankan Gao  1 , Huamin Zhong  1 , Yongqiang Xie  1 , Bingshao Liang  1 , Wenjing Ji  2 , Haiying Liu  3
Affiliations

Automated classification of group B Streptococcus into different clonal complexes using MALDI-TOF mass spectrometry

Lianfen Huang et al. Front Mol Biosci. .

Abstract

Objectives: To evaluate the performance of Matrix-Assisted Laser Desorption/Ionization Time-of Flight Mass Spectra (MALDI-TOF MS) for automated classification of GBS (Group B Streptococcus) into five major CCs (clonal complexes) during routine GBS identification.

Methods: MALDI-TOF MS of 167 GBS strains belonging to five major CCs (CC10, CC12, CC17, CC19, CC23) were grouped into a reference set (n = 67) and a validation set (n = 100) for the creation and evaluation with GBS CCs subtyping main spectrum (MSP) and MSP-M using MALDI BioTyper and ClinProTools. GBS CCs subtyping MSPs-M was generated by resetting the discriminative peaks of GBS CCs subtyping MSP according to the informative peaks from the optimal classification model of five major CCs and the contribution of each peak to the model created by ClinProTools.

Results: The PPV for the GBS CCs subtyping MSP-M was greater than the subtyping MSP for CC10 (99.21% vs. 93.65%), but similar for CC12 (79.55% vs. 81.06%), CC17 (93.55% vs. 94.09%), and CC19 (92.59% vs. 95.37%), and lower for CC23 (66.67% vs. 83.33%).

Conclusion: MALDI-TOF MS could be a promising tool for the automated categorization of GBS into 5 CCs by both CCs subtyping MSP and MSP-M, GBS CCs subtyping MSP-M is preferred for the accurate prediction of CCs with highly discriminative peaks.

Keywords: classification; classification clean version group B Streptococcus; classification group B Streptococcus; clone complexes (CCs); group B streptococcus; main spectrum (MSP); matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
The flow chart of the study design.
FIGURE 2
FIGURE 2
Averaged spectra plots showing the presence or absence of corresponding peak biomarkers for matrix-assisted laser desorption ionization time-of flight mass spectrometry discrimination of the five main Group B streptococcus clonal complexes in the optimal genetic algorithm model. CC10 (red), CC12 (pink), CC17 (green); CC19 (blue), CC23 (yellow). x-axis shows the mass per charge ratio values (m/z) and y-axis indicates the intensities of peaks expressed in arbitrary intensity units.
FIGURE 3
FIGURE 3
Two-dimensional peak distribution diagrams displaying the pair distribution for the best separating peaks in the optimal genetic algorithm model. The ellipses respresent the standard deviation of the class average of the peak areas/intensities. CC10 (red), CC12 (green), CC17 (blue); CC19 (yellow), CC23 (pink). The peak numbers and m/z values are indicated on the x- and y-axes, respectively.
FIGURE 4
FIGURE 4
The preliminary procedure for the recognizing of GBS CCs by GBS mass spectra according to the established GBS CCs subtyping MSP and MSP-M models.
See this image and copyright information in PMC

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