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Review
. 2023 Dec 20:2:1323387.
doi: 10.3389/frtra.2023.1323387. eCollection 2023.

Review of machine perfusion studies in vascularized composite allotransplant preservation

Çağdaş Duru  1 , Felor Biniazan  1 , Nina Hadzimustafic  1   2 , Andrew D'Elia  1   2 , Valentina Shamoun  1 , Siba Haykal  1   3
Affiliations
Review

Review of machine perfusion studies in vascularized composite allotransplant preservation

Çağdaş Duru et al. Front Transplant. .

Abstract

The applications of Vascularized composite allotransplantation (VCA) are increasing since the first successful hand transplantation in 1998. However, the abundance of muscle tissue makes VCA's vulnerable to ischemia-reperfusion injury (IRI), which has detrimental effects on the outcome of the procedure, restricting allowable donor-to-recipient time and limiting its widespread use. The current clinical method is Static cold storage (SCS) and this allows only 6 h before irreversible damage occurs upon reperfusion. In order to overcome this obstacle, the focus of research has been shifted towards the prospect of ex-vivo perfusion preservation which already has an established clinical role in solid organ transplants especially in the last decade. In this comprehensive qualitative review, we compile the literature on all VCA machine perfusion models and we aim to highlight the essentials of an ex vivo perfusion set-up, the different strategies, and their associated outcomes.

Keywords: ex-vivo limb perfusion; ex-vivo perfusion; hand transplantation; ischemia reperfusion (I/R) injury; vascularized composite allograft (VCA).

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
The essentials of ex vivo limb perfusion setup. Created with BioRender.com.
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References

    1. Carrel A, Lindbergh CA. The culture of whole organs. Science. (1935) 81(2112):621–3. 10.1126/science.81.2112.621 - DOI - PubMed
    1. Lindbergh CA. An apparatus for the culture of whole organs. J Exp Med. (1935) 62(3):409–31. 10.1084/jem.62.3.409 - DOI - PMC - PubMed
    1. Belzer FO, Ashby BS, Gulyassy PF, Powell M. Successful seventeen-hour preservation and transplantation of human-cadaver kidney. N Engl J Med. (1968) 278(11):608–10. 10.1056/NEJM196803142781108 - DOI - PubMed
    1. Starzl TE, Groth CG, Brettschneider L, Penn I, Fulginiti VA, Moon JB, et al. Orthotopic homotransplantation of the human liver. Ann Surg. (1968) 168(3):392–415. 10.1097/00000658-196809000-00009 - DOI - PMC - PubMed
    1. Robinson WR, Peters RH, Zimmermann J. The effects of body size and temperature on metabolic rate of organisms. Can J Zool. (1983) 61(2):281–8. 10.1139/z83-037 - DOI

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