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. 2024:2821:225-236.
doi: 10.1007/978-1-0716-3914-6_18.

Assessment of the Antigen-Binding Capacity and Separation of Extracellular Vesicles Coated with Antigen-Specific Antibody Light Chains

Katarzyna Nazimek  1 , Krzysztof Bryniarski  2
Affiliations

Assessment of the Antigen-Binding Capacity and Separation of Extracellular Vesicles Coated with Antigen-Specific Antibody Light Chains

Katarzyna Nazimek et al. Methods Mol Biol. 2024.

Abstract

Many researchers are interested in the possibility of manipulating the targeting specificity of extracellular vesicles (EVs) for their use as physiological delivery vehicles for drugs and bioactive molecules. Our studies demonstrated the possibility of directing EVs toward the desired acceptor cell by coating them with antigen-specific antibody light chains. Here, we describe the methods for detection of the presence of antibody light chains on the EV surface, proving their ability to specifically bind the antigen and for separating the antigen-binding EV subpopulation.

Keywords: Antibody light chains; Antigen binding; Antigen-affinity chromatography; Cell targeting by extracellular vesicles; Cytometry; ELISA; Exosomes; Extracellular vesicles; Separation of extracellular vesicles; Specificity of antigen binding.

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