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. 1985 Oct;164(1):470-2.
doi: 10.1128/jb.164.1.470-472.1985.

Isolation of a dihydrofolate reductase-deficient mutant of Escherichia coli

S Singer, R Ferone, L Walton, L Elwell

Isolation of a dihydrofolate reductase-deficient mutant of Escherichia coli

S Singer et al. J Bacteriol. 1985 Oct.

Abstract

A strain of Escherichia coli was isolated in which dihydrofolate reductase was not detected by an enzyme assay or by competition for antibody. This strain requires methionine, glycine, a purine, and thymidine for growth in addition to the auxotrophic requirements of the parent strain. It was found to be useful as a recipient of plasmids harboring dihydrofolate reductase genes.

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