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. 2024 Jun 17:12:260.
doi: 10.12688/f1000research.126044.3. eCollection 2023.

Cloning and characterization of the LvCTL genes encoding C-type lectin from white-leg shrimp ( Litopenaeus vannamei)

Tran Vinh Phuong  1   2 , Xuan Huy Nguyen  1 , Nguyen Quang Lich  1 , Ngoc Nguyen Tran  2 , Phuoc Ngoc Nguyen  2 , Nguyen Quang Linh  3
Affiliations

Cloning and characterization of the LvCTL genes encoding C-type lectin from white-leg shrimp ( Litopenaeus vannamei)

Tran Vinh Phuong et al. F1000Res. .

Abstract

Background: Lectins are carbohydrate-binding protein domains. The C-type lectin designates a requirement for calcium for binding. Proteins contain C-type lectin domains that have a diverse range of functions, including cell-cell adhesion, immune response to pathogens, and apoptosis. This study aimed to investigate the characters of LvCTL-encoding genes from white-leg shrimp ( Litopenaeus vannamei) in Central Vietnam.

Methods: Two PCR products (LvCTL3 and LvCTL4) were cloned and sequenced. The structure and characterization of LvCTL proteins were predicted using bioinformatics tools.

Results: The results showed that the LvCTL3 gene was 444 nucleotides in length and 98.87% similar to the published LvCTL3 gene (accession number: KF156943). The polypeptide sequence had 147 amino acids, which were 97.28% identical to the reference sequence (AGV68681) and the LvCTL4 gene had a length of 417 nucleotides and homology of 99.52% compared to the published gene (KM387560). The deduced polypeptide sequence had 138 amino acids, and was 100% similar to the reference sequence (AKA64754). The LvCTL3 had a molecular weight of 16.91 kDa and an isoelectric point (pI) of 4.66, while LvCTL4 had 15.75 and 4.58 kDa, respectively. The structure prediction results showed that LvCTL3 and LvCTL4 had one domain (CTLD), LvCTL3 had two α helices and nine β sheets, and LvCTL4 had two α helices and eight β sheets.

Conclusions: Our results provide essential information for the heterologous expression and biosynthesis production of C-type lectins.

Keywords: C-type lectin; LvCTL genes; gene encoding; white leg shrimp..

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Conflict of interest statement

No competing interests were disclosed.

Figures

Figure 1.
Figure 1.. PCR product LvCTL genes on agarose gel.
M: DNA ladder (GeneRuler TM 1 kb DNA Ladder, Thermo Scientific, USA). NC: Negative control. 1-5: PCR product with cDNA of LvCTL3-LvCTL4- LvAV-LvCTLD-LvLdlrCTL genes. 6-7: PCR product of LvCTL3 gene from 2 different colonies. 8-9: PCR product of LvCTL4 gene from 2 different colonies.
Figure 2.
Figure 2.. The similarity of the nucleotide sequences of the isolated LvCTL3 gene and the published LvCTL3 gene (KF156943).
Figure 3.
Figure 3.. The similarity of the nucleotide sequences of the isolated LvCTL4 gene and the published LvCTL4 gene (KM387560).
Figure 4.
Figure 4.. The similarity of the deduced amino acid of the isolated from LvCTL3 gene and the published LvCTL3 sequence (AGV68681).
Figure 5.
Figure 5.. The similarity of the deduced amino acid of the isolated from LvCTL4 gene and the published LvCTL4 sequence (AKA64754).
Figure 6.
Figure 6.. Taxonomy tree LvCTL3 and LvCTL4 deduced amino acid sequence compared to other published CTLs.
Figure 7.
Figure 7.. The predicted of LvCTL3 structure.
Figure 8.
Figure 8.. The predicted of LvCTL4 structure.
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References

    1. Ha DTT, Tuan LK, Lan PTN, et al. : Cloning and sequencing laccase 3 (folac3) from Fusarium oxysporum. Hue University Journal of Science: Natural Science. 2018;127(1C):1–10. 10.26459/hueuni-jns.v127i1C.4892 - DOI
    1. He S, et al. : Molecular characterization of LvAV in response to white spot syndrome virus infection in the Pacific white shrimp ( Litopenaeus vannamei). Dev. Comp. Immunol. 2015;51(1):48–55. 10.1016/j.dci.2015.02.020 - DOI - PubMed
    1. Janeway CA, Jr, Medzhitov R: Innate immune recognition. Annu. Rev. Immunol. 2002;20:197–216. 10.1146/annurev.immunol.20.083001.084359 - DOI - PubMed
    1. Junkunlo K, Prachumwat A, Tangprasittipap A, et al. : A novel lectin domain-containing protein (LvCTLD) associated with the response of the whiteleg shrimp Penaeus (Litopenaeus) vannamei to the yellow head virus (YHV). Dev. Comp. Immunol. 2012;37(3-4):334–341. 10.1016/j.dci.2011.12.010 - DOI - PubMed
    1. Kelley LA, Mezulis S, Yates CM, et al. : The Phyre2 web portal for protein modeling, prediction and analysis. Nat. Protoc. 2015;10(6):845–858. 10.1038/nprot.2015.053 - DOI - PMC - PubMed

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