Copper nanoparticles loaded gelatin/ polyvinyl alcohol/ guar gum-based 3D printable multimaterial hydrogel for tissue engineering applications

Abstract

Hydrogels are becoming increasingly significant in tissue engineering because of their numerous benefits, including biocompatibility, biodegradability, and their ability to provide a supportive structure for cell proliferation. This study presents the synthesis and characterization of a new multimaterial hydrogel with 3D-printing capabilities composed of copper nanoparticle-reinforced gelatin, polyvinyl alcohol (PVA), and guar gum-based biomaterials intended for tissue engineering applications. Combining CuNPs aims to enhance the hydrogel's antibacterial properties, mechanical strength, and bioactivity, which are essential for successful tissue regeneration. Hydrogels are chemically cross-linked with glyoxal and analyzed through different assessments to examine the compressive behavior, surface morphology, sorbing capacity, biocompatibility, thermal stability, and degradation properties. The results demonstrated that including CuNPs significantly improved the hydrogel's compressive modulus (4.18 MPa) for the hydrogel with the CuNPs and provided better antibacterial activity against common pathogens with controlled degradation. All the hydrogels exhibited a lower coefficient of friction, which was below 0.1. In vitro cell culture studies using chondrocytes indicated that the CuNPs-loaded hydrogel supported cell proliferation and growth of chondrogenic genes such as collagen type II (COL2) and aggrecan (ACAN). The biocompatibility and enhanced mechanical properties of the multimaterial hydrogel make it a promising candidate for developing customized, patient-specific tissue engineering scaffolds.

Keywords: 3D printing; Gelatin; Guar gum; Hydrogel.