Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review

Quantitation of PEG on PEGylated Gold Nanoparticles Using Reversed Phase High Performance Liquid Chromatography and Charged Aerosol Detection: Version 1.0

Jeffrey D. Clogston, Mackensie C. Smith
In: National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet]. Bethesda (MD): National Cancer Institute (US); 2005 May. NCL Method PCC-16.
2017 Oct.
Free Books & Documents
Review

Quantitation of PEG on PEGylated Gold Nanoparticles Using Reversed Phase High Performance Liquid Chromatography and Charged Aerosol Detection: Version 1.0

Jeffrey D. Clogston et al.
Free Books & Documents

Excerpt

This protocol describes a method for the quantitation of polyethylene glycol (PEG) in PEGylated colloidal gold nanoparticles using reversed phase high performance liquid chromatography (RP-HPLC) with charged aerosol detection (CAD). The method can be used to calculate the total PEG on the nanoparticle, as well as the bound and free unbound PEG fractions after a simple centrifugation step. This is a significant distinction as the bound PEG fraction affects biocompatibility, circulation time, and overall nanoparticle efficacy. PEG quantitation can be achieved through two methods, one involving dissolution of colloidal gold nanoparticles by potassium cyanide (KCN) and the other by displacement of PEG by dithiothreitol (DTT). The methods outlined herein were applied to 30 nm colloidal gold grafted with 20 kDa PEG, but they can be easily adapted to any size colloidal gold nanoparticle and PEG chain length.

The method development was previously published in ref. (1) and the detailed protocol is published in ref. (2).

PubMed Disclaimer

References

    1. Smith MC, Crist RM, Clogston JD, & McNeil SE (2015) Quantitative analysis of PEG-functionalized colloidal gold nanoparticles using charged aerosol detection. Anal Bioanal Chem 407(13):3705–3716. - PubMed
    1. Smith MC & Clogston JD (2017) PEG Quantitation Using Reversed Phase High Performance Liquid Chromatography and Charged Aerosol Detection. Characterization of Nanoparticles Intended for Drug Delivery, Methods in Molecular Biology, ed McNeil SE (Humana Press; ), 2nd Ed Vol 1682. - PubMed
    1. Albanese A, Tang PS, & Chan WCW (2012) The Effect of Nanoparticle Size, Shape, and Surface Chemistry on Biological Systems. Annu Rev Biomed Eng 14:1–16. - PubMed
    1. Harris JM & Chess RB (2003) Effect of pegylation on pharmaceuticals. Nat Rev Drug Discov 2(3):214–221. - PubMed
    1. Jokerst JV, Lobovkina T, Zare RN, & Gambhir SS (2011) Nanoparticle PEGylation for imaging and therapy. Nanomedicine-Uk 6(4):715–728. - PMC - PubMed

LinkOut - more resources