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Review

Detection of Bacterial Contamination Using Tryptic Soy Broth Agar Plates: Version 3

Barry W. Neun  1 , Timothy M. Potter  1 , Marina A. Dobrovolskaia  1
In: National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet]. Bethesda (MD): National Cancer Institute (US); 2005 May. NCL Method STE-2.3.
2020 Sep.
Affiliations
Review

Detection of Bacterial Contamination Using Tryptic Soy Broth Agar Plates: Version 3

Barry W. Neun et al.

Excerpt

This protocol describes a procedure for quantitative determination of microbial contamination in a nanoparticle preparation. Nanoparticle samples, along with controls, are spread on the surface of agar and growth of bacterial colonies is monitored after 72 hr of incubation. The intended purpose of this assay is to avoid introduction of microbial contamination into in vitro cell cultures and in vivo animal studies utilizing the test-nanomaterial, as microbial contamination will confound the results of these tests. This assay is not intended to certify the material as sterile.

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