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Review

LLC-PK1 Kidney Cytotoxicity Assay: Version 1.3

Stephan T. Stern, Pavan P. Adiseshaiah, Timothy M. Potter
In: National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet]. Bethesda (MD): National Cancer Institute (US); 2005 May. NCL Method GTA-1.
2020 Apr.
Free Books & Documents
Review

LLC-PK1 Kidney Cytotoxicity Assay: Version 1.3

Stephan T. Stern et al.
Free Books & Documents

Excerpt

This protocol describes the cytotoxicity testing of nanoparticle formulations porcine proximal tubule cells (LLC-PK1), as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes two methods for estimation of cytotoxicity, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) release [–2].

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References

    1. ISO 10993-5, Biological evaluation of medical devices: Part 5, Tests for in vitro cytotoxicity.
    1. F1903 – 98, Standard Practice for Testing for Biological Responses to Particles in vitro.
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    1. Decker, T. & Lohmann-Matthes, M.L. (1988) J. Immunol Methods 15:61–69. - PubMed
    1. Korzeniewski, C. & Callewaert, D.M. (1983) J. Immunol Methods 64:313–320. - PubMed

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