Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Sep 1;230(2):345-51.
doi: 10.1042/bj2300345.

Anomeric specificity of hexokinase and glucokinase activities in liver and insulin-producing cells

A Sener, M H Giroix, S P Dufrane, W J Malaisse

Anomeric specificity of hexokinase and glucokinase activities in liver and insulin-producing cells

A Sener et al. Biochem J. .

Abstract

Conflicting data have been reported concerning the anomeric specificity of glucokinase. In the present study, liver hexokinase (Km for D-glucose 0.4 mM) displayed a higher affinity for but lower Vmax. with alpha- than with beta-D-glucose. The velocity of the reaction catalysed by liver glucokinase was higher with with beta- than with alpha-D-glucose, whatever the glucose concentration. The apparent Km of glucokinase was somewhat lower, however, with alpha- than with beta-D-glucose. Comparable results were obtained for the high-Km glucokinase-like enzymic activity present in normal pancreatic islets or insulin-producing tumoral cells. These results suggest that the anomeric specificity of glucokinase cannot account for the higher rate of glycolysis found in islets exposed to alpha- as distinct from beta-D-glucose.

PubMed Disclaimer

References

    1. J Biol Chem. 1976 Oct 10;251(19):5936-43 - PubMed
    1. J Biol Chem. 1976 Mar 25;251(6):1731-7 - PubMed
    1. J Biochem. 1978 Oct;84(4):993-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 Jan;80(1):85-9 - PubMed
    1. FEBS Lett. 1982 Dec 27;150(2):325-8 - PubMed

Publication types

LinkOut - more resources