Inhibition of cell-surface neutral protease of Ehrlich ascites tumor cells by potassium p-(3,3-dimethyl-1-triazeno) benzoate
- PMID: 3902023
Item in Clipboard
Inhibition of cell-surface neutral protease of Ehrlich ascites tumor cells by potassium p-(3,3-dimethyl-1-triazeno) benzoate
Biochem Int.
1985 Aug.
Abstract
Ehrlich ascites tumour (EAT) cells possess a trypsin-like neutral protease on the cell surface. The antimetastatic triazene drug potassium p-(3,3-dimethyl-1-triazeno) benzoate (DM-COOK) inhibits this neutral protease, and also trypsin. Incubation of EAT cells with human erythrocytes (ratio of 1 to 5) at 37 degrees C for 18 h caused haemolysis of 28.8% of the erythrocytes. Addition of millimolar concentrations of DM-COOK to a fixed quantity (2.5 X 10(8)) of EAT cells caused a dose-related inhibition of haemolysis. DM-COOK was strongly bound to EAT cells and could not be removed by repeated washing.
Similar articles
-
Selectivity of the antimetastatic and cytotoxic effects of 1-p-(3,3-dimethyl-1-triazeno)benzoic acid potassium salt (+/-)-1,2-di(3,5-dioxopiperazin-1-yl)propane, and cyclophosphamide in mice bearing Lewis lung carcinoma.Cancer Res. 1981 Jun;41(6):2524-8. Cancer Res. 1981. PMID: 7237446
-
Activity and inhibition by cytotoxic and antimetastatic drugs of cathepsin B-like cysteine proteinase in transplantable leukemias in mice.Anticancer Res. 1987 May-Jun;7(3 Pt B):343-6. Anticancer Res. 1987. PMID: 3307599
-
beta-Naphthylamidase activity of the cell surface of Ehrlich ascites cells. Reversible control of enzyme activity by metal ions and thiols.Br J Cancer. 1981 Nov;44(5):709-16. doi: 10.1038/bjc.1981.257. Br J Cancer. 1981. PMID: 6797455 Free PMC article.
-
Inhibitors of proteases as anticancer drugs.Neoplasma. 2005;52(3):185-92. Neoplasma. 2005. PMID: 15875078 Review.
-
Further inhibition studies on guanidinobenzoatase, a trypsin-like enzyme associated with tumour cells.J Enzyme Inhib. 1987;1(3):187-201. doi: 10.3109/14756368709020116. J Enzyme Inhib. 1987. PMID: 3334244 Review.