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. 2024 Nov 1;109(11):3771-3775.
doi: 10.3324/haematol.2024.285266.

A targeted gene signature stratifying mediastinal gray zone lymphoma into classical Hodgkin lymphoma-like or primary mediastinal B-cell lymphoma-like subtypes

Grazia Gargano  1 , Maria Carmela Vegliante  2 , Flavia Esposito  3 , Susanna A Pappagallo  2 , Elena Sabattini  4 , Claudio Agostinelli  5 , Stefano A Pileri  6 , Valentina Tabanelli  6 , Maurilio Ponzoni  7 , Luisa Lorenzi  8 , Fabio Facchetti  8 , Arianna Di Napoli  9 , Marco Lucioni  10 , Marco Paulli  10 , Lorenzo Leoncini  11 , Stefano Lazzi  11 , Stefano Ascani  12 , Giuseppina Opinto  13 , Gian Maria Zaccaria  14 , Giacomo Volpe  2 , Paolo Mondelli  2 , Antonella Bucci  2 , Laura Selicato  3 , Antonio Negri  2 , Giacomo Loseto  2 , Felice Clemente  2 , Anna Scattone  15 , Alfredo F Zito  15 , Luca Nassi  16 , Nicoletta Del Buono  3 , Attilio Guarini  2 , Sabino Ciavarella  17
Affiliations

A targeted gene signature stratifying mediastinal gray zone lymphoma into classical Hodgkin lymphoma-like or primary mediastinal B-cell lymphoma-like subtypes

Grazia Gargano et al. Haematologica. .
No abstract available

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Figures

Figure 1.
Figure 1.
Schematic overview of the study design. The methodological workflow included three different phases and independent cohorts of patients. The gene selection phase related to the training/ testing set was conducted in-silico (light blue), while the validation (light green) and MGZL assignment phases (red) were completed on a real-life set of cases (24 out of 28 centrally revised MGZL passed the quality control for the final study phase). CHL: classical Hodgkin lymphoma; PMBL: primary mediastinal B-cell lymphoma; GZL: gray zone lymphoma; MGZL: mediastinal gray zone lymphoma.
Figure 2.
Figure 2.
Identification of a molecular signature distinguishing classical Hodgkin lymphoma and primary mediastinal B-cell lymphoma. Schematic overview of the CIBERSORTx analysis. We applied CIBERSORTx for digital purification of a bulk mixture matrix (50 classical Hodgkin lymphomas and 31 primary mediastinal B-cell lymphomas as the training set) using a customized signature matrix of 24-cell types, composed of two tumor cytotypes and 22 tumor microenvironment (TME) cells. Tumor and TME compartments were run as merged classes to purify the gene expression profile for both components. The t-distributed stochastic neighbor embedding visualization of the two purified tumor and TME gene expression profiles are depicted on the right side. CHL: classical Hodgkin lymphoma; PMBL: primary mediastinal B-cell lymphoma; BC: B cells; TC: T cells; NK: natural killer cells; Mo: macrophages; DC: dendritic cells; GEP: gene expression profiles; t-SNE: t-distributed stochastic neighbor embedding.
See this image and copyright information in PMC

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