Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Oct;518(1):355-360.
doi: 10.1134/S1607672924600362. Epub 2024 Jul 18.

Study of Radiosensitivity and Induction of Radiation Adaptive Response in Peripheral Blood Lymphocytes of Patients with Oncological Diseases Using the Micronuclear Test

V E Balakin  1 , O M Rozanova  2 , N S Strelnikova  3 , E N Smirnova  2 , T A Belyakova  2   4
Affiliations

Study of Radiosensitivity and Induction of Radiation Adaptive Response in Peripheral Blood Lymphocytes of Patients with Oncological Diseases Using the Micronuclear Test

V E Balakin et al. Dokl Biochem Biophys. 2024 Oct.

Abstract

Radiosensitivity to low and medium doses of X-ray radiation and the ability to induce a radiation adaptive response (RAR) of lymphocytes during in vitro irradiation of peripheral blood of patients with cancer were studied. The criterion for cytogenetic damage was the frequency of micronuclei (MN) in cytochalasin-blocked binucleate lymphocytes in culture. It was found that the spontaneous level of cytogenetic damage in the lymphocytes of patients was 2.6 times higher than in healthy volunteers, and there was also significant interindividual variability in values compared to the control cohort. There were no differences in mean values for radiosensitivity to low and medium doses of X-ray between the study groups. There was no correlation between the spontaneous level of MN in lymphocytes and the radiosensitivity of individuals in both groups. RAR was induced with the same frequency and to the same extent in lymphocytes from both patients and healthy individuals.

Keywords: cancer; human lymphocytes; ionizing radiation; micronuclei; radiation adaptive response; radiosensitivity.

PubMed Disclaimer

References

REFERENCES

    1. Pelevina, I.I., Petushkova, V.V., Biryukov, V.A., et al., The “radiation-induced non-targeted effects” and their role in human cell response to low radiation forcing, Radiats. Biol. Radioecol., 2019, vol. 59, no. 3, pp. 261–273. https://doi.org/10.1134/S086980311903010X - DOI
    1. Kogarko, I.N., Akleev, A.V., Petushkova, V.V., et al., Adaptive response as a criterion for assessing individual human radiosensitivity. Review, Radiats. Risk, 2022, vol. 31, no. 1, pp. 93–104. https://doi.org/10.21870/0131-3878-2022-31-1-93-104 - DOI
    1. Rödel, F., Frey, B., Gaipl, U., et al., Modulation of inflammatory immune reactions by low-dose ionizing radiation: molecular mechanisms and clinical application, Curr. Med. Chem., 2012, vol. 19, no. 12, pp. 1741–1750. https://doi.org/10.2174/092986712800099866 - DOI - PubMed
    1. Balakin, V.E., Zaichkina, S.I., Rozanova, O.M., et al., Low-dose ionizing radiation inhibits the aging-related rise in accumulation of spontaneous cytogenetic abnormalities: the genome-stabilizing effect, Dokl. Biol. Sci., 2000, vol. 374, pp. 488–490. - PubMed
    1. Ghiassi-Nejad, M., Mortazavi, S.M., Cameron, J.R., et al., Very high background radiation areas of Ramsar, Iran: preliminary biological studies, Health Phys., 2002, vol. 82, no. 1, pp. 87–93. https://doi.org/10.1097/00004032-200201000-00011 - DOI - PubMed

LinkOut - more resources