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Comment
. 2024 Aug 14;15(8):e0111624.
doi: 10.1128/mbio.01116-24. Epub 2024 Jul 19.

Experimental uncoupling of hosts and endosymbionts

Honglin Feng  1 , Alex C C Wilson  2
Affiliations
Comment

Experimental uncoupling of hosts and endosymbionts

Honglin Feng et al. mBio. .

Abstract

Many organisms harbor heritable bacterial symbionts that offer context-specific benefits to their hosts. In some of these symbioses, symbionts live inside host cells as endosymbionts. Studying the biology of endosymbiosis is challenging because it is hard to independently cultivate hosts and endosymbionts. A recent study, using a simple defined growth medium at ambient temperature, established an axenic culture of the pea aphid's heritable bacterial endosymbiont, Candidatus Fukatsuia symbiotica (G. P. Maeda, M. K. Kelly, A. Sundar, and N. A. Moran, mBio 15:e03253-23, 2024, https://doi.org/10.1128/mbio.03253-23). Notably, the monoculture was capable of host recolonization, was stably transmitted, and returned similar host phenotypes to those observed in native infections. This advance in uncoupling the cultivation of an endosymbiont and its host opens avenues for genetic manipulation of the endosymbiont that will facilitate hypothesis-driven work to explore the mechanisms of host-endosymbiont biology and potentially facilitate the development of symbiont-mediated practical-application biotechnologies.

Keywords: Fukatsuia; axenic culture; endosymbiosis.

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Conflict of interest statement

The authors declare no conflict of interest.

Comment on

References

    1. Maeda GP, Kelly MK, Sundar A, Moran NA. 2024. Intracellular defensive symbiont is culturable and capable of transovarial, vertical transmission. mBio 15:e03253-23. doi:10.1128/mbio.03253-23 - DOI - PMC - PubMed
    1. Manzano-Marín A, Szabó G, Simon J-C, Horn M, Latorre A. 2017. Happens in the best of subfamilies: establishment and repeated replacements of co‐obligate secondary endosymbionts within Lachninae aphids. Environ Microbiol 19:393–408. doi:10.1111/1462-2920.13633 - DOI - PubMed
    1. Chevignon G, Boyd BM, Brandt JW, Oliver KM, Strand MR. 2018. Culture-facilitated comparative genomics of the facultative symbiont Hamiltonella defensa. Genome Biol Evol 10:786–802. doi:10.1093/gbe/evy036 - DOI - PMC - PubMed
    1. Patel V, Chevignon G, Manzano-Marín A, Brandt JW, Strand MR, Russell JA, Oliver KM. 2019. Cultivation-assisted genome of Candidatus Fukatsuia symbiotica; the enigmatic “X-type” symbiont of aphids. Genome Biol Evol 11:3510–3522. doi:10.1093/gbe/evz252 - DOI - PMC - PubMed
    1. Nadal-Jimenez P, Griffin JS, Davies L, Frost CL, Marcello M, Hurst GDD. 2019. Genetic manipulation allows in vivo tracking of the life cycle of the son‐killer symbiont, Arsenophonus nasoniae, and reveals patterns of host invasion, tropism and pathology. Environ Microbiol 21:3172–3182. doi:10.1111/1462-2920.14724 - DOI - PMC - PubMed

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